Differentiation between Xanthomonas campestris pv. oryzae, Xanthomonas campestris pv. oryzicola and the Bacterial 'Brown Blotch' Pathogen on Rice by Numerical Analysis of Phenotypic Features and Protein Gel Electrophoregrams
| Autor: | J. De Ley, Karel Kersters, C. M. Vera Cruz, F. Gosselé, Paul Segers, M. Van Den Mooter, J. Swings |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
DNA
Bacterial Base Composition Xanthomonas biology Pseudomonas Nucleic Acid Hybridization food and beverages Oryza Pseudomonas fluorescens Ribosomal RNA biology.organism_classification Microbiology Xanthomonas campestris RNA Bacterial Phenotype RNA Ribosomal Azomonas Electrophoresis Polyacrylamide Gel Flavobacterium Plant Diseases Plant Proteins Pseudomonadaceae |
| Zdroj: | Microbiology. 130:2983-2999 |
| ISSN: | 1465-2080 1350-0872 |
| DOI: | 10.1099/00221287-130-11-2983 |
| Popis: | Thirty-five Xanthomonas campestris pv. oryzae, fourteen X. campestris pv. oryzicola strains and six 'brown blotch' pathogens of rice, all of different geographical origin, were studied by numerical analysis of 133 phenotype features and gel electrophoregrams of soluble proteins, %G + C determinations and DNA:rRNA hybridizations. The following conclusions were drawn. (i) The Xanthomonas campestris pathovars oryzae and oryzicola display clearly distinct protein patterns on polyacrylamide gels and can be differentiated from each other by four phenotype tests. (ii) Both pathovars are indeed members of Xanthomonas which belongs to a separate rRNA branch of the second rRNA superfamily together with the rRNA branches of Pseudomonas fluorescens, Marinomonas, Azotobacter, Azomonas and Frateuria. (iii) 'Brown blotch' strains are considerably different from X. campestris pv. oryzae and oryzicola. They are not members of the genus Xanthomonas, but are more related to the generically misnamed. Flavobacterium capsulatum, Pseudomonas paucimobilis, Flavobacterium devorans and 'Pseudomonas azotocolligans' belonging in the fourth rRNA superfamily. (iv) No correlation was found between the virulence, pathogenic groups or geographical distribution of X. campestris pv. oryzae or oryzicola strains and any phenotypic or protein electrophoretic property or clustering. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|