PKCε is a negative regulator of PVAT-derived vessel formation
| Autor: | Maria Luisa Arcari, Giuliana Gobbi, A. Dei Cas, Domenico Corradi, Marcello Vitale, Antonio Nouvenne, Daniela Galli, Giovanna Bucci, Cecilia Carubbi, Prisco Mirandola, Elena Masselli |
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| Rok vydání: | 2015 |
| Předmět: |
Vascular Endothelial Growth Factor A
Adventitia Cellular differentiation Down-Regulation Neovascularization Physiologic Adipose tissue Smad Proteins Protein Kinase C-epsilon Biology Coronary Restenosis Mice Vasculogenesis Downregulation and upregulation medicine Animals Myocytes Cardiac Progenitor cell Cells Cultured Progenitor Calcium-Binding Proteins Microfilament Proteins Endothelial Cells Cell Differentiation Cell Biology Anatomy Actins Cell biology Enzyme Activation Platelet Endothelial Cell Adhesion Molecule-1 Vascular endothelial growth factor A medicine.anatomical_structure Adipose Tissue p21-Activated Kinases |
| Zdroj: | Experimental Cell Research. 330:277-286 |
| ISSN: | 0014-4827 |
| Popis: | Rationale Vessel formation is a crucial event in tissue repair after injury. Thus, one assumption of innovative therapeutic approaches is the understanding of its molecular mechanisms. Notwithstanding our knowledge of the role of Protein Kinase C epsilon (PKCe) in cardio-protection and vascular restenosis, its role in vessel progenitor differentiation remains elusive. Objective Given the availability of PKCe pharmacological modulators already tested in clinical trials, the specific aim of this study is to unravel the role of PKCe in vessel progenitor differentiation, with implications in vascular pathology and vasculogenesis. Methods and results Mouse Peri-Vascular Adipose Tissue (PVAT) was used as source of mesenchymal vessel progenitors. VEGF-induced differentiation of PVAT cells down-regulates both PKCe and p-PAK1 protein expression levels. PKCe overexpression and activation: i) reduced the expression levels of SMA and PECAM in endothelial differentiation of PVAT cells; ii) completely abrogated tubules formation in collagen gel assays; iii) increased the expression of p-PAK1. Conclusion PKCe negatively interferes with vessel progenitor differentiation via interaction with PAK-1. |
| Databáze: | OpenAIRE |
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