Determination of a new antibacterial agent (Ro 23-9424) by multidimensional high-performance liquid chromatography with ultraviolet detection and direct plasma injection
| Autor: | Roger W. Blain, Alice J. Szuna |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Detection limit
Chromatography Molecular Structure Elution Hydrolysis Metabolite Reproducibility of Results Cefotaxime General Chemistry High-performance liquid chromatography chemistry.chemical_compound Fleroxacin Anti-Infective Agents Drug Stability chemistry Chromatography Gel Humans Spectrophotometry Ultraviolet Sample preparation Steady state (chemistry) Quantitative analysis (chemistry) Chromatography High Pressure Liquid Fluoroquinolones Antibacterial agent |
| Zdroj: | Journal of Chromatography B: Biomedical Sciences and Applications. 620:211-216 |
| ISSN: | 0378-4347 |
| DOI: | 10.1016/0378-4347(93)80006-p |
| Popis: | Analysis of a new antibacterial agent, Ro 23-9424 (I), in plasma has been complicated by the fact that its metabolite, fleroxacin (II), is formed not only in vivo, but also nonenzymatically by the hydrolysis of the ester bond of I. In order to minimize sample preparation time and possible hydrolysis during sample preparation, a high-performance liquid chromatographic procedure was developed which features direct injection of plasma and multidimensional chromatography. The first dimension size-exclusion separation allows plasma proteins to elute with the column void volume. The second dimension reversed-phase column provides a high-resolution separation dependent upon the hydrophobicity of the sample species. With a 5-microliters injection, the limit of quantitation of the method is 0.35 microgram/ml for I and 0.27 microgram/ml for II. The method was used to determine steady state plasma vs. time profiles for I and II from 750 mg i.v. doses of I administered twice daily. |
| Databáze: | OpenAIRE |
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