Neuron-specific knockouts indicate the importance of network communication to Drosophila rhythmicity
| Autor: | Madelen M. Díaz, Jason Xin, Michael Rosbash, Matthias Schlichting |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Circadian clock
Cell Communication 0302 clinical medicine Drosophila Proteins Biology (General) Feedback Physiological Gene Editing Neurons 0303 health sciences D. melanogaster General Neuroscience Brain General Medicine Period Circadian Proteins Darkness Clock network CLOCK medicine.anatomical_structure Basic-Leucine Zipper Transcription Factors Drosophila melanogaster Medicine Research Article circadian rhythm Light Signal Transduction QH301-705.5 Science Period (gene) Biology General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Circadian Clocks medicine Animals Cell Lineage Circadian rhythm Gene knockout 030304 developmental biology General Immunology and Microbiology activity Neuropeptides network communication Gene Expression Regulation nervous system Neuron CRISPR-Cas Systems Nerve Net Neuroscience 030217 neurology & neurosurgery Transcription Factors |
| Zdroj: | eLife eLife, Vol 8 (2019) |
| DOI: | 10.1101/639146 |
| Popis: | Animal circadian rhythms persist in constant darkness and are driven by intracellular transcription-translation feedback loops. Although these cellular oscillators communicate, isolated mammalian cellular clocks continue to tick away in darkness without intercellular communication. To investigate these issues in Drosophila, we assayed behavior as well as molecular rhythms within individual brain clock neurons while blocking communication within the ca. 150 neuron clock network. We also generated CRISPR-mediated neuron-specific circadian clock knockouts. The results point to two key clock neuron groups: loss of the clock within both regions but neither one alone has a strong behavioral phenotype in darkness; communication between these regions also contributes to circadian period determination. Under these dark conditions, the clock within one region persists without network communication. The clock within the famous PDF-expressing s-LNv neurons however was strongly dependent on network communication, likely because clock gene expression within these vulnerable sLNvs depends on neuronal firing or light. |
| Databáze: | OpenAIRE |
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