DLK-1 as a Marker to Distinguish Unrestricted Somatic Stem Cells and Mesenchymal Stromal Cells in Cord Blood
| Autor: | Constanze Wiek, Gesine Kögler, Amelie Pia Houben, Petra Reinecke, Teja Falk Radke, Helmut Hanenberg, Thomas Krenz, Stefanie Geyh, Peter Wernet, Anja Buchheiser, Simone Maria Kluth |
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| Rok vydání: | 2010 |
| Předmět: |
Cellular differentiation
CD34 Biology medicine Humans Gene Silencing Stem Cells Calcium-Binding Proteins Mesenchymal stem cell Membrane Proteins Cell Differentiation Mesenchymal Stem Cells Cell Biology Hematology Fetal Blood Cell biology Haematopoiesis medicine.anatomical_structure Gene Expression Regulation Cord blood Immunology Intercellular Signaling Peptides and Proteins Bone marrow Stromal Cells Stem cell Biomarkers Developmental Biology Adult stem cell |
| Zdroj: | Stem Cells and Development. 19:1471-1483 |
| ISSN: | 1557-8534 1547-3287 |
| DOI: | 10.1089/scd.2010.0070 |
| Popis: | In addition to hematopoietic stem cells, cord blood (CB) also contains different nonhematopoietic CD45-, CD34- adherent cell populations: cord blood mesenchymal stromal cells (CB MSC) that behave almost like MSC from bone marrow (BM MSC) and unrestricted somatic stem cells (USSC) that differentiate into cells of all 3 germ layers. Distinguishing between these populations is difficult due to overlapping features such as the immunophenotype or the osteogenic and chondrogenic differentiation pathway. Functional differences in the differentiation potential suggest different developmental stages or different cell populations. Here we demonstrate that the expression of genes and the differentiation toward the adipogenic lineage can discriminate between these 2 populations. USSC, including clonal-derived cells lacking adipogenic differentiation, strongly expressed#x03B4;-like 1/preadipocyte factor 1 (DLK-1/PREF1) correlating with high proliferative potential, while CB MSC were characterized by a strong differentiation toward adipocytes correlating with a weak or negative DLK-1/PREF1 expression. Constitutive overexpression of DLK-1/PREF1 in CB MSC resulted in a reduced adipogenic differentiation, whereas silencing of DLK-1 in USSC resulted in adipogenic differentiation. |
| Databáze: | OpenAIRE |
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