Effect of Iontophoresis on Topical Delivery of Doxorubicin-Loaded Solid Lipid Nanoparticles
| Autor: | Auro Nomizo, Luciana Mattoso Pires de Campos Araújo, Stephânia Fleury Taveira, Franciane Marquele-Oliveira, Danielle Cristine Almeida Silva de Santana, Renata Fonseca Vianna Lopez |
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| Rok vydání: | 2014 |
| Předmět: |
Materials science
Administration Topical Sus scrofa Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering In Vitro Techniques Pharmacology Permeability Drug Delivery Systems Cell Line Tumor Solid lipid nanoparticle Electric Impedance polycyclic compounds Stratum corneum medicine Animals Humans General Materials Science Doxorubicin Cytotoxicity Electrodes Skin Iontophoresis organic chemicals Melanoma NEOPLASIAS CUTÂNEAS technology industry and agriculture Penetration (firestop) medicine.disease Lipids body regions carbohydrates (lipids) medicine.anatomical_structure Drug delivery Nanoparticles Electroosmosis medicine.drug |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1550-7033 |
| DOI: | 10.1166/jbn.2014.1834 |
| Popis: | The combination of iontophoresis with solid lipid nanoparticles (SLNs) for targeting drug delivery to the epidermis has not been explored. The goal of this paper was to study the influence of iontophoresis on the penetration of doxorubicin (DOX) delivered in SLNs (DOX-SLNs). We measured the contribution of electroosmotic flow to the transport of DOX, and the accumulation of DOX in the stratum corneum (SC) and in the viable epidermis was determined. In addition, we evaluated the cytotoxicity of DOX-SLNs against skin cancer cells. Iontophoresis of unloaded SLNs decreased the electroosmotic flow by a factor of 5 and increased the skin resistance. Nevertheless, iontophoresis of DOX-SLNs increased DOX delivery to the viable epidermis, with 56% of all DOX penetrating this skin layer. Only 26% of the drug was retained in the SC. In contrast, passive delivery retained 43% of DOX in the SC and 26% in the viable epidermis. DOX-SLNs increased DOX cytotoxicity against melanoma cells by 50%. These results suggest the use of DOX-SLN iontophoresis in the topical treatment of skin cancer. |
| Databáze: | OpenAIRE |
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