Identification of Four ATP-Binding Cassette Transporter Genes in Cnaphalocrocis medinalis and Their Expression in Response to Insecticide Treatment
| Autor: | Dong-Qiong Fei, Dong Yu, Yan Ma, Jia-Ping Xu, Hai-Zhong Yu, Xue-yang Wang, Wan-Ling Wang, Shang-Zhi Zhang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Insecticides ATP-binding cassette transporter Moths Insecticide Resistance Transcriptome 03 medical and health sciences Transcription (biology) Hemolymph Animals insecticide detoxification Gene biology Cnaphalocrocis Gene Expression Profiling mRNA expression General Medicine biology.organism_classification Cnaphalocrocis medinalis Gene expression profiling 030104 developmental biology Biochemistry Larva Insect Science Inactivation Metabolic ATP-Binding Cassette Transporters Chlorpyrifos ABC transporter Research Article |
| Zdroj: | Journal of Insect Science |
| ISSN: | 1536-2442 |
| DOI: | 10.1093/jisesa/iex017 |
| Popis: | The ATP-binding cassette (ABC) transporters belong to a superfamily of genes involved in the transport of specific molecules across lipid membranes, as well as insecticide resistance, present in all living organisms. In this study, we combined the Cnaphalocrocis medinals transcriptome database with a bioinformatics approach to identify four C. medinals ABCs (CmABCs), including CmABCG1, CmABCG4, CmABCC2 and CmABCC3. Tissue expression analysis showed that these genes had a tissue-specific expression pattern. CmABCG1 had significantly higher expression in the haemolymph and head compared to the other tissues. The expression of CmABCG4, CmABCC2 and CmABCC3 was highest in the midgut, followed by expression in the fat body. The developmental stage expression analysis showed that CmABCG1, CmABCG4, CmABCC2 and CmABCC3 were mainly expressed in adults. The transcription of CmABCG1, CmABCG4 and CmABCC2 was significantly induced by chlorpyrifos. Taken together, the results of our study provided useful information for understanding of the detoxification system of C. medinalis. |
| Databáze: | OpenAIRE |
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