Augmentation of skeletal tissue formation in impaction bone grafting using vaterite microsphere biocomposites
| Autor: | Stephen Mann, David W. Green, Dominic M. Walsh, Stuart A. Lanham, Richard O.C. Oreffo, B.J.R.F. Bolland, Janos M. Kanczler |
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| Rok vydání: | 2009 |
| Předmět: |
Stromal cell
Materials science medicine.medical_treatment Osteocalcin Biophysics Bone Matrix Biocompatible Materials Bone Marrow Cells Enzyme-Linked Immunosorbent Assay Bioengineering Bone grafting Calcium Carbonate Biomaterials Extracellular matrix Osteogenesis In vivo Vaterite medicine Humans Cells Cultured Bone Transplantation Tissue Engineering biology Impaction Microspheres In vitro Mechanics of Materials Ceramics and Composites biology.protein Collagen Stromal Cells Tomography X-Ray Computed Biomedical engineering |
| Zdroj: | Biomaterials. 30:1918-1927 |
| ISSN: | 0142-9612 |
| Popis: | The development of particulate bone void fillers with added biological function to augment skeletal tissue formation will lead to improved efficacy in bone replacement surgery. We demonstrate the potential for vaterite microsphere biocomposites to augment bone matrix formation within an in vivo model for impaction bone grafting seeded with human bone marrow stromal cells. In vitro tests demonstrate the significance of vaterite microspheres in the activation and promotion of 3D skeletal tissue formation. Further in vitro experiments using functionalized microspheres with surface integrated RGD peptide activate co-cultured skeletal populations in pellets and promote secretion of extracellular matrix collagens and human osteocalcin. Specific temporal release of entrapped RNase A was successfully demonstrated using these specialized microspheres with integrated magnetic beads, which physically disrupted the inorganic macrostructure. These studies demonstrate that bio-inspired calcium carbonate microspheres augment in vivo bone formation in impaction bone grafting. Such microspheres with added biological functionality offer innovative therapeutic approaches to activate skeletal populations and enhance bone formation with reparative implications for hard tissues. |
| Databáze: | OpenAIRE |
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