Scalable Spatial-Spectral Multiplexing of Single-Virus Detection Using Multimode Interference Waveguides
| Autor: | M. A. Stott, Damla Ozcelik, Alexandra Stambaugh, Aaron R. Hawkins, Holger Schmidt, Joshua W. Parks, Aadhar Jain |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
lcsh:Medicine
02 engineering and technology Signal Multiplexing Sensitivity and Specificity Article law.invention 020210 optoelectronics & photonics law 0202 electrical engineering electronic engineering information engineering Multiplex Sensitivity (control systems) lcsh:Science Fluorescent Dyes Physics Spatial Analysis Multidisciplinary Staining and Labeling business.industry lcsh:R Equipment Design Microfluidic Analytical Techniques 021001 nanoscience & nanotechnology Chip Spatial multiplexing Spectrometry Fluorescence Viruses Optoelectronics lcsh:Q Photonics 0210 nano-technology business Telecommunications Waveguide |
| Zdroj: | Scientific Reports, Vol 7, Iss 1, Pp 1-8 (2017) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-017-12487-0 |
| Popis: | Simultaneous detection of multiple pathogens and samples (multiplexing) is one of the key requirements for diagnostic tests in order to enable fast, accurate and differentiated diagnoses. Here, we introduce a novel, highly scalable, photonic approach to multiplex analysis with single virus sensitivity. A solid-core multimode interference (MMI) waveguide crosses multiple fluidic waveguide channels on an optofluidic chip to create multi-spot excitation patterns that depend on both the wavelength and location of the channel along the length of the MMI waveguide. In this way, joint spectral and spatial multiplexing is implemented that encodes both spatial and spectral information in the time dependent fluorescence signal. We demonstrate this principle by using two excitation wavelengths and three fluidic channels to implement a 6x multiplex assay with single virus sensitivity. High fidelity detection and identification of six different viruses from a standard influenza panel is reported. This multimodal multiplexing strategy scales favorably to large numbers of targets or large numbers of clinical samples. Further, since single particles are detected unbound in flow, the technique can be broadly applied to direct detection of any fluorescent target, including nucleic acids and proteins. |
| Databáze: | OpenAIRE |
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