Coculture with intraocular lens material-activated macrophages induces an inflammatory phenotype in lens epithelial cells
| Autor: | Cameron K Postnikoff, Maud Gorbet, Sara Molladavoodi, Robert Pintwala |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Materials science
medicine.medical_treatment Biomedical Engineering Biocompatible Materials Intraocular lens Cell Line law.invention Flow cytometry Biomaterials Cataracts Confocal microscopy law Lens Crystalline Materials Testing Cell Adhesion medicine Humans Polymethyl Methacrylate Macrophage Polyhydroxyethyl Methacrylate Inflammation Lenses Intraocular medicine.diagnostic_test Macrophages Transdifferentiation Epithelial Cells Anatomy Capsule Opacification Macrophage Activation Intercellular Adhesion Molecule-1 medicine.disease Actins Coculture Techniques eye diseases Cell biology Phenotype medicine.anatomical_structure Cell culture Lens (anatomy) Posterior Capsule of the Lens Cytokines sense organs Inflammation Mediators Reactive Oxygen Species Hydrophobic and Hydrophilic Interactions |
| Zdroj: | Journal of Biomaterials Applications. 29:1119-1132 |
| ISSN: | 1530-8022 0885-3282 |
| Popis: | Cataracts are the leading cause of blindness worldwide, requiring surgical implantation of an intraocular lens. Despite evidence of leukocyte ingress into the postoperative lens, few studies have investigated the leukocyte response to intraocular lens materials. A novel coculture model was developed to examine macrophage activation by hydrophilic acrylic (poly(2-hydroxyethyl methacrylate)) and hydrophobic acrylic (polymethylmethacrylate) commercial intraocular lens. The human monocytic cell line THP-1 was differentiated into macrophages and cocultured with human lens epithelial cell line (HLE-B3) with or without an intraocular lens for one, two, four, or six days. Using flow cytometry and confocal microscopy, expression of the macrophage activation marker CD54 (intercellular adhesion molecule-1) and production of reactive oxygen species via the fluorogenic probe 2′,7′-dichlorodihydrofluorescein diacetate were examined in macrophages. α-Smooth muscle actin, a transdifferentiation marker, was characterized in lens epithelial cells. The poly(2-hydroxyethyl methacrylate) intraocular lens prevented adhesion but induced significant macrophage activation ( p |
| Databáze: | OpenAIRE |
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