Detection of Mycobacterium tuberculosis complex with Real Time PCR: Comparison of different primer-probe sets based on the IS6110 element
| Autor: | Sije Mulder, Arnold Catsburg, J. Schirm, G. T. Noordhoek, Adri G. M. van der Zanden, Hans Wilke, Paul H. M. Savelkoul, Ludo Oostendorp |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
DNA
Bacterial Microbiology (medical) biology Mycobacterium tuberculosis Computational biology biology.organism_classification Polymerase Chain Reaction Sensitivity and Specificity Microbiology Molecular biology law.invention Real-time polymerase chain reaction Mycobacterium tuberculosis complex law DNA Transposable Elements Tuberculosis Primer (molecular biology) DNA Probes Molecular Biology Polymerase chain reaction DNA Primers |
| Zdroj: | Journal of Microbiological Methods. 66:177-180 |
| ISSN: | 0167-7012 |
| DOI: | 10.1016/j.mimet.2005.12.003 |
| Popis: | The sensitivity and specificity to detect Mycobacterium tuberculosis complex of four Real Time PCR primer-probe sets was compared. Three sets targeted nearly the same location on the IS6110 sequence and set 4 targeted a location 200 bp downstream on IS6110. Real Time PCR's with sets 1, 2 and 3 were carried out with co-amplification of a modified target as an internal amplification control. By testing identical DNA samples it was shown that small changes in primer and probe sequences result in differences in the performance of the assays, regarding analytical sensitivity and specificity. |
| Databáze: | OpenAIRE |
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