Interaction of alphaviruses with human peripheral leukocytes: in vitro replication of Venezuelan equine encephalomyelitis virus in monocyte cultures
| Autor: | Helen V. Miller, Neil H. Levitt, Robert R. Edelman |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Neutrophils
viruses Microgram Immunology Cell Separation Biology medicine.disease_cause Microbiology Encephalitis Virus Western Equine Monocytes Virus Cell Line medicine Humans Lymphocytes Cells Cultured Western equine encephalitis virus Monocyte virus diseases medicine.disease Virology In vitro Peripheral Infectious Diseases medicine.anatomical_structure Cell culture Encephalitis Virus Eastern Equine Parasitology Arboviruses Encephalitis Research Article |
| Zdroj: | Infection and Immunity. 24:642-646 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.24.3.642-646.1979 |
| Popis: | Human peripheral blood leukocytes (PBL) were examined for their ability to support growth of several group A arboviruses in vitro. Cells were refractory to infection with eastern (EEE) and western (WEE) equine encephalitis viruses, whereas Venezuelan equine encephalomyelitis (VEE) virus was shown to infect and replicate to a substantially high titer. When PBL were fractionated into purified subpopulations, only the monocytes were susceptible to predictive VEE virus infection. Lymphocytes treated 24 h before virus inoculation with phytohemagglutinin (10 microgram/ml) were capable of propagating significant amounts of VEE virus. A monocytic cell line, J-111, was also susceptible to infection with VEE, EEE, and WEE viruses, whereas a lymphocytic cell line, Raji, was refractory. Additional information on the participation of PBL during human infection with these viruses may add considerably to our understanding of their differing pathogenicities and clinical pictures. |
| Databáze: | OpenAIRE |
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