Methylation of the estrogen receptor gene is associated with aging and atherosclerosis in the cardiovascular system
| Autor: | Mark S. Sussman, Pamela Ouyang, Wendy S. Post, Emily E. Milliken, Calvin C. Wilhide, Alan W. Heldman, Jean Pierre J. Issa, Pascal J. Goldschmidt-Clermont |
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| Rok vydání: | 1999 |
| Předmět: |
Male
Aging medicine.medical_specialty Physiology Estrogen receptor Coronary Disease Biology Cardiovascular System Muscle Smooth Vascular Physiology (medical) Internal medicine medicine.artery medicine Humans Saphenous Vein Heart Atria Mammary Arteries Receptor Aorta Cells Cultured Vascular disease Estrogen Receptor alpha Promoter Methylation DNA Methylation Middle Aged medicine.disease Blotting Southern Endocrinology Receptors Estrogen DNA methylation Female Endothelium Vascular Cardiology and Cardiovascular Medicine Estrogen receptor alpha |
| Zdroj: | Cardiovascular Research. 43:985-991 |
| ISSN: | 0008-6363 |
| Popis: | Objective: Methylation of the promoter region of the estrogen receptor gene alpha ( ER α ) occurs as a function of age in human colon, and results in inactivation of gene transcription. In this study, we sought to determine whether such age-related methylation occurs in the cardiovascular system, and whether it is associated with atherosclerotic disease. Methods: We used Southern blot analysis to determine the methylation state of the ER α gene in human right atrium, aorta, internal mammary artery, saphenous vein, coronary atherectomy samples, as well as cultured aortic endothelial cells and smooth muscle cells. Results: An age related increase in ER α gene methylation occurs in the right atrium (range 6 to 19%, R =0.36, P |
| Databáze: | OpenAIRE |
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