Retinol-Binding Protein 4 Induces Cardiomyocyte Hypertrophy by Activating TLR4/MyD88 Pathway
| Autor: | Xiang Lu, Zhengxia Liu, Lian-Sheng Wang, Lin Zhang, Hao Wang, Qin Yang, Wei Gao, Yang Cao, Ji-Zhang Bao |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty Glucose uptake medicine.medical_treatment Blotting Western Adipose tissue Cardiomegaly Inflammation 030204 cardiovascular system & hematology Polymerase Chain Reaction Losartan Muscle hypertrophy Mice 03 medical and health sciences 0302 clinical medicine Endocrinology Insulin resistance Internal medicine Adipocytes medicine Animals Myocytes Cardiac Cells Cultured Original Research Retinol binding protein 4 biology Angiotensin II Insulin medicine.disease Mice Inbred C57BL Toll-Like Receptor 4 Oxidative Stress 030104 developmental biology Heart failure Myeloid Differentiation Factor 88 biology.protein medicine.symptom Reactive Oxygen Species Retinol-Binding Proteins Plasma Signal Transduction |
| Zdroj: | Endocrinology. 157:2282-2293 |
| ISSN: | 1945-7170 0013-7227 |
| DOI: | 10.1210/en.2015-2022 |
| Popis: | Insulin resistance plays a major role in the development and progression of cardiac hypertrophy and heart failure. Heart failure in turn promotes insulin resistance and increases the risk for diabetes. The vicious cycle determines significant mortality in patients with heart failure and diabetes. However, the underlying mechanisms for the vicious cycle are not fully elucidated. Here we show that circulating levels and adipose expression of retinol-binding protein 4 (RBP4), an adipokine that contributes to systemic insulin resistance, were elevated in cardiac hypertrophy induced by transverse aortic constriction and angiotensin-II (Ang-II) infusion. Ang-II increased RBP4 expression in adipocytes, which was abolished by losartan, an Ang-II receptor blocker. The elevated RBP4 in cardiac hypertrophy may have pathophysiological consequences because RBP4 increased cell size, enhanced protein synthesis, and elevated the expression of hypertrophic markers including Anp, Bnp, and Myh7 in primary cardiomyocytes. Mechanistically, RBP4 induced the expression and activity of toll-like receptor 4 (TLR4) and myeloid differentiation primary response gene 88 (MyD88) in cardiomyocytes, resulting in enhanced inflammation and reactive oxygen species production. Inhibition or knockdown of the TLR4/MyD88 pathway attenuated inflammatory and hypertrophic responses to RBP4 stimulation. Importantly, RBP4 also reduced the expression of glucose transporter-4 and impaired insulin-stimulated glucose uptake in cardiomyocytes. This impairment was ameliorated in cardiomyocytes from TLR4 knockout mice. Therefore, RBP4 may be a critical modulator promoting the vicious cycle of insulin resistance and heart failure by activating TLR4/MyD88-mediated inflammatory pathways. Potentially, lowering RBP4 might break the vicious cycle and improve both insulin resistance and cardiac hypertrophy. |
| Databáze: | OpenAIRE |
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