Variability of antioxidant properties, catechins, caffeine, L-theanine and other amino acids in different plant parts of Azorean Camellia sinensis
| Autor: | José Baptista, Madalena Motta, Massimo F. Marcone, Lisete S. Paiva, Elisabete Lima |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
RP-HPLC analysis
Antioxidant Total phenolics medicine.medical_treatment lcsh:TX341-641 Phenylalanine Review Article Total flavonoids Applied Microbiology and Biotechnology Tea antioxidants chemistry.chemical_compound Rutin Catechin profiles medicine Camellia sinensis Gallic acid Food science Catechin Profiles Plant stem Rp-Hplc Analysis Methionine lcsh:TP368-456 L-Theanine/Caffeine Content Chemistry Free amino acid profiles Internode Waste Valorization lcsh:Food processing and manufacture Total Phenolics Free Amino Acid Profiles L-theanine/caffeine content Tea Antioxidants Total Flavonoids Caffeine Internode waste valorization lcsh:Nutrition. Foods and food supply Food Science Biotechnology |
| Zdroj: | Current Research in Food Science Current Research in Food Science, Vol 3, Iss, Pp 227-234 (2020) |
| ISSN: | 2665-9271 |
| Popis: | During Camellia sinensis tea processing, manufacturers usually remove the internodes, which are classified as waste. This study presents the first determination of plant part contribution, particularly internodes, to green tea quality, in order to find the best blend to maximize impact on human health. Catechins, caffeine and free amino acid (FAA) profiles were determined by RP-HPLC/DAD, total phenolics (TPC) and total flavonoids (TFC) by Folin-Ciocalteu and colorimetric methodologies, respectively, and antioxidant activities by free radical-scavenging activity (FRSA), ferric-reducing antioxidant power (FRAP) and ferrous ion-chelating (FIC) methods. Individual esterified catechins content decreased as follows: epicatechin-3-gallate > epigallocatechin-3-gallate ≫ gallocatecin-3-gallate, and epicatechin derivatives content ranged from 63.91 to 91.22% of total catechins. Caffeine content was higher in internodes. L-theanine, histidine, asparagine, phenylalanine, glutamic acid and methionine were the major FAAs, and internodes contained the highest amounts of L-theanine and histidine (17 and 13.73 mg/g of sample, respectively). TPC ranged from 201.51 to 265.48 mg gallic acid equivalents/g dry extract (DE) and TFC ranged from 23.84 to 72.02 mg rutin equivalents/g DE. Internodes presented the lowest FRSA (EC50 = 6.10–13.50 μg/mL), FRAP (EC50 = 5.70–11.40 μg/mL) and FIC activity (36.96–79.21%). Bud presented the highest FRSA and FRAP, and bud+1st+2ndleaves + internodes the highest FIC activity. The results revealed the potential contribution of the internodes to green tea quality and, consequently, to human health. Graphical abstract Image 1 Highlights • Metabolites variability is observed in different parts of Azorean Camellia sinensis. • Camellia sinensis plant parts are good sources of polyphenols (catechins). • Internodes are not useless tea plant waste but a valuable tea quality component. • Theanine is the major amino acid in Camellia sinensis particularly in internodes. • Addition of internodes to the tea leaves have significative impact on human health. |
| Databáze: | OpenAIRE |
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