RIMA-dependent nuclear accumulation of IYO triggers auxin-irreversible cell differentiation in arabidopsis
| Autor: | Maite Sanmartín, Jose J. Sánchez-Serrano, Enrique Rojo, Dolf Weijers, Mary Paz González-García, Ramon Contreras, Alfonso Muñoz, Bert De Rybel, Michael Sauer, Silvina Mangano |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Monoamine Oxidase Inhibitors Arabidopsis thaliana Cellular differentiation organogenesis Arabidopsis Biochemie Plant Science Biochemistry Ciencias Biológicas 03 medical and health sciences Gene Expression Regulation Plant ddc:570 Transcriptional regulation Cell differentiation Life Science heterocyclic compounds Lateral root formation Institut für Biochemie und Biologie Research Articles Genetics biology Indoleacetic Acids Arabidopsis Proteins fungi food and beverages Cell Differentiation Cell Biology Meristem Bioquímica y Biología Molecular biology.organism_classification Plants Genetically Modified Cell biology stem cell Pericycle 030104 developmental biology Nuclear transport EPS CIENCIAS NATURALES Y EXACTAS |
| Zdroj: | The Plant Cell 29 (2017) 3 The Plant Cell, 29(3), 575-588 |
| ISSN: | 1040-4651 |
| Popis: | The transcriptional regulator MINIYO (IYO) is essential and rate-limiting for initiating cell differentiation in Arabidopsis thaliana Moreover, IYO moves from the cytosol into the nucleus in cells at the meristem periphery, possibly triggering their differentiation. However, the genetic mechanisms controlling IYO nuclear accumulation were unknown, and the evidence that increased nuclear IYO levels trigger differentiation remained correlative. Searching for IYO interactors, we identified RPAP2 IYO Mate (RIMA), a homolog of yeast and human proteins linked to nuclear import of selective cargo. Knockdown of RIMA causes delayed onset of cell differentiation, phenocopying the effects of IYO knockdown at the transcriptomic and developmental levels. Moreover, differentiation is completely blocked when IYO and RIMA activities are simultaneously reduced and is synergistically accelerated when IYO and RIMA are concurrently overexpressed, confirming their functional interaction. Indeed, RIMA knockdown reduces the nuclear levels of IYO and prevents its prodifferentiation activity, supporting the conclusion that RIMA-dependent nuclear IYO accumulation triggers cell differentiation in Arabidopsis. Importantly, by analyzing the effect of the IYO/RIMA pathway on xylem pole pericycle cells, we provide compelling evidence reinforcing the view that the capacity for de novo organogenesis and regeneration from mature plant tissues can reside in stem cell reservoirs. Fil: Muñoz, Alfonso. Consejo Superior de Investigaciones Cientificas; España Fil: Mangano, Silvina. Consejo Superior de Investigaciones Cientificas; España. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Bioquímicas de Buenos Aires. Fundación Instituto Leloir. Instituto de Investigaciones Bioquímicas de Buenos Aires; Argentina Fil: González-García, Mary Paz. Consejo Superior de Investigaciones Cientificas; España Fil: Contreras, Ramón. Consejo Superior de Investigaciones Cientificas; España Fil: Sauer, Michael. Universitat Potsdam; Alemania. Consejo Superior de Investigaciones Cientificas; España Fil: De Rybel, Bert. University of Ghent; Bélgica. Wageningen University; Países Bajos Fil: Weijers, Dolf. Wageningen University; Países Bajos Fil: Sánchez Serrano, José Juan. Consejo Superior de Investigaciones Cientificas; España Fil: Sanmartín, Maite. Consejo Superior de Investigaciones Cientificas; España Fil: Rojo, Enrique. Consejo Superior de Investigaciones Cientificas; España |
| Databáze: | OpenAIRE |
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