Vaccination of ducks with recombinant outer membrane protein (OmpA) and a 41 kDa partial protein (P45N′) of Riemerella anatipestifer
| Autor: | Kim Lee Chua, Hai-Meng Tan, Joachim Frey, Charlene J Fernandez, Sumathi Subramaniam, Bin Huang, Jimmy Kwang, Hilda Loh |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
DNA
Bacterial Serotype Recombinant Fusion Proteins animal diseases Immunoblotting Molecular Sequence Data Virulence Enzyme-Linked Immunosorbent Assay Polymerase Chain Reaction Microbiology law.invention law Animals Serotyping Poultry Diseases Glutathione Transferase Base Sequence General Veterinary biology Vaccination Riemerella anatipestifer General Medicine Antibodies Bacterial Virology Fusion protein Specific Pathogen-Free Organisms Molecular Weight Ducks Gram-Negative Aerobic Rods and Cocci Porin biology.protein Recombinant DNA bacteria Antibody Gram-Negative Bacterial Infections Bacterial outer membrane Bacterial Outer Membrane Proteins |
| Zdroj: | Veterinary Microbiology. 84:219-230 |
| ISSN: | 0378-1135 |
| Popis: | The generation of protective immunity against Riemerella anatipestifer infection in ducks were investigated by immunizations with recombinant glutathione sulfatransferase (GST) fusion's proteins of OmpA, a 42kDa major outer membrane protein, and P45N', a 41kDa N-terminal fragment of a newly identified 45kDa potential surface protein from R. anatipestifer. The DNA encoding OmpA and P45N' were isolated from R. anatipestifer serotype 15 (field strain 110/89) and serotype 19 (reference strain 30/90), respectively. Immunoblotting and ELISA results showed that the purified recombinant proteins induced the production of antibodies in immunized ducks. However, neither was protective against subsequent challenge with the virulent serotype 15 strain, 34/90. All the five ducks immunized with formalinized R. anatipestifer strain 34/90 survived the challenge with the homologous strain whereas six out of seven ducks in the non-immunized control group died within a week following the challenge. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect