Determination of Polyhexamethylene Biguanide Utilizing a Glucose Oxidase Enzymatic Reaction
| Autor: | Kohei Uematsu, Hajime Katano, Akihito Shinozaki |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
medicine.drug_class
Biguanides 02 engineering and technology Biosensing Techniques 01 natural sciences Colorimetry (chemical method) Analytical Chemistry Catalysis chemistry.chemical_compound Glucose Oxidase Limit of Detection medicine Glucose oxidase Ferricyanides Detection limit biology Biguanide 010401 analytical chemistry Osmolar Concentration Cationic polymerization 021001 nanoscience & nanotechnology 0104 chemical sciences Contact lens chemistry biology.protein Ferricyanide Aspergillus niger 0210 nano-technology Nuclear chemistry |
| Zdroj: | Analytical sciences : the international journal of the Japan Society for Analytical Chemistry. 35(9) |
| ISSN: | 1348-2246 |
| Popis: | Polyhexamethylene biguanide (PHMB) is a cationic disinfectant widely used for personal-care products and for sanitizers in swimming pools. This paper describes a promotion effect of PHMB on a glucose oxidase (GOx) enzymatic reaction with ferricyanide ion and its analytical application. The promotion effect arose from a polyion complex formation between polycationic PHMB and polyanionic GOx. The promoted GOx reaction was analyzed by Michaelis-Menten equation, and the Michalis constant and catalytic constant were estimated to be 240 μM and 31 s-1, respectively. Utilizing the promotion effect, PHMB was successfully determined in the range of 0.05 to 0.40 ppm, and the detection limit was calculated to be 0.027 ppm. The visual detection and semi-determination of PHMB with the same concentration level were also possible. As an application, the method was applied to the determination of PHMB in a contact lens detergent and its model solution. |
| Databáze: | OpenAIRE |
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