Circulating osteocyte‐related biomarkers (vitamin D, sclerostin, dickkopf-1), hepcidin, and oxidative stress markers in early breast cancer: Their impact in disease progression and outcome

Autor: Madeha M. Zakhary, Doaa W. Maximous, Reham I El-Mahdy, Abeer A Mokhtar, Mahmoud I. El Dosoky
Rok vydání: 2020
Předmět:
Adult
0301 basic medicine
medicine.medical_specialty
Endocrinology
Diabetes and Metabolism
Clinical Biochemistry
Breast Neoplasms
medicine.disease_cause
Osteocytes
Biochemistry
Pathogenesis
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Endocrinology
Breast cancer
Hepcidins
Hepcidin
Internal medicine
medicine
Vitamin D and neurology
Humans
Vitamin D
Molecular Biology
Adaptor Proteins
Signal Transducing
Aged
biology
business.industry
Vitamins
Cell Biology
Middle Aged
medicine.disease
Oxidative Stress
030104 developmental biology
chemistry
DKK1
Tumor progression
Case-Control Studies
030220 oncology & carcinogenesis
Disease Progression
biology.protein
Intercellular Signaling Peptides and Proteins
Molecular Medicine
Sclerostin
Female
business
Biomarkers
Oxidative stress
Zdroj: The Journal of Steroid Biochemistry and Molecular Biology. 204:105773
ISSN: 0960-0760
DOI: 10.1016/j.jsbmb.2020.105773
Popis: Breast cancer (BC) is a major concern to female health worldwide. We assessed the circulating osteocyte-related biomarkers, hepcidin, and oxidative stress status among early-stage BC patients in aspects of clinical severity and impact on the outcome. The study incorporated 73 patients categorized into 57 early-stage BC and 16 benign breast diseases and 30 healthy controls. Serum 25-hydroxyvitamin D [25(OH)D], sclerostin (SOST), dickkopf-1(DKK1), and hepcidin were measured using ELISA, while, serum oxidative stress markers were assessed by spectrophotometry. Our results show that patients with BC showed significant increase in the mean levels of DKK1, SOST, hepcidin, and LPER and significant decrease in the mean levels of 25(OH)D, SOD, GPx, and Hb when compared with controls and benign breast diseases. Significantly higher DKK1, hepcidin, and SOD levels among benign breast diseases were found in comparison to control group. There were significantly lower levels of 25(OH)D, SOD, and Hb and significantly higher levels of SOST, DKK1, hepcidin, No, and LPER with advanced grade. Lower levels of 25(OH)D, SOD and higher levels of SOST, hepcidin were observed with increasing the malignant stage. Reduced levels of 25(OH)D, and SOD were significantly associated with poor prognosis and were strong predictors among BC. There were significant negative correlations between 25(OH)D with LPER, SOST, and hepicidin. We conclude that low 25(OH)D, high SOST, DKK1, and hepcidin, and dysregulated oxidative stress could be helpful in early detection and assessment of BC. 25(OH)D, and SOD were the most relevant to tumor progression and prognosis which indicate a significant role in the BC pathogenesis and could be promising targets in management. Our research paves the way to disrupt vicious circle between these biomarkers to obtain the best care of BC.
Databáze: OpenAIRE
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