Chloroplast to chromoplast transition in tomato fruit: spectral confocalmicroscopy analyses of carotenoids and chlorophylls in isolated plastids and time-lapse recording on intact live tissue
| Autor: | Zhengguo Li, Cristina Barsan, Wanping Bian, Christian Chervin, Alain Latché, Jean-Claude Pech, Alain Jauneau, Isabel Egea |
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| Přispěvatelé: | Centre National de la Recherche Scientifique - CNRS (FRANCE), Chongqing University (CHINA), Institut National Polytechnique de Toulouse - INPT (FRANCE), Institut National de la Recherche Agronomique - INRA (FRANCE), Université Toulouse III - Paul Sabatier - UT3 (FRANCE), Génomique et Biotechnologie des Fruits (GBF), Institut National de la Recherche Agronomique (INRA)-École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Université Fédérale Toulouse Midi-Pyrénées, Chongqing University, Centre National de la Recherche Scientifique (CNRS), National Natural Science Foundation of China [31071798], Midi-Pyrenees Regional Council [CR07003760], Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE) |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
0106 biological sciences
Chlorophyll Chloroplasts [SDV]Life Sciences [q-bio] Plant Science tomato 01 natural sciences Chloroplast Pigment fluorescence Tomato law.invention 03 medical and health sciences chemistry.chemical_compound Pigment Solanum lycopersicum Confocal microscopy law Chromoplast Génétique des plantes Plastids Plastid Carotenoid 030304 developmental biology chemistry.chemical_classification 0303 health sciences Microscopy Confocal biology fungi food and beverages Original Articles pigment fluorescence biology.organism_classification Carotenoids Botanique chemistry Biochemistry Microscopy Fluorescence confocal visual_art Fruit Confocal visual_art.visual_art_medium Solanum chromoplast 010606 plant biology & botany |
| Zdroj: | Annals of Botany Annals of Botany, Oxford University Press (OUP), 2011, 108 (2), pp.291-297. ⟨10.1093/aob/mcr140⟩ |
| ISSN: | 0305-7364 1095-8290 |
| Popis: | Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699; International audience; Background and Aims There are several studies suggesting that tomato (Solanum lycopersicum) chromoplasts arise from chloroplasts, but there is still no report showing the fluorescence of both chlorophylls and carotenoids in an intermediate plastid, and no video showing this transition phase. Methods Pigment fluorescence within individual plastids, isolated from tomato fruit using sucrose gradients, was observed at different ripening stages, and an in situ real-time recording of pigment fluorescence was performed on live tomato fruit slices. Key results At the mature green and red stages, homogenous fractions of chloroplasts and chromoplasts were obtained, respectively. At the breaker stage, spectral confocal microscopy showed that intermediate plastids contained both chlorophylls and carotenoids. Furthermore, an in situ real-time recording (a) showed that the chloroplast to chromoplast transition was synchronous for all plastids of a single cell; and (b) confirmed that all chromoplasts derived from pre-existing chloroplasts. Conclusions These results give details of the early steps of tomato chromoplast biogenesis from chloroplasts, with the formation of intermediate plastids containing both carotenoids and chlorophylls. They provide information at the sub-cellular level on the synchronism of plastid transition and pigment changes. |
| Databáze: | OpenAIRE |
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