Scalable Labeling for Cytoarchitectonic Characterization of Large Optically Cleared Human Neocortex Samples
| Autor: | Anna Schueth, Ralf A. W. Galuske, Andreas Herrler, Alard Roebroeck, Sven Hildebrand |
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| Přispěvatelé: | RS: FPN CN 11, Multiscale Imaging of Brain Connectivity, Anatomie & Embryologie, RS: FHML non-thematic output |
| Rok vydání: | 2019 |
| Předmět: |
Adult
0301 basic medicine Optics and Photonics ORGANS Materials science Confocal lcsh:Medicine Neocortex Context (language use) Article law.invention 03 medical and health sciences Imaging Three-Dimensional 0302 clinical medicine law Cortex (anatomy) medicine Microtome Humans BRAIN lcsh:Science Microscopy Confocal Multidisciplinary Staining and Labeling Light-sheet microscopy lcsh:R Microtomy Human brain PLANE ILLUMINATION MICROSCOPY 030104 developmental biology medicine.anatomical_structure IDISCO Cytoarchitecture Light sheet fluorescence microscopy lcsh:Q 030217 neurology & neurosurgery Biomedical engineering |
| Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-10 (2019) Scientific Reports, 9(1):10880. Nature Publishing Group Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-019-47336-9 |
| Popis: | Optical clearing techniques and light sheet microscopy have transformed fluorescent imaging of rodent brains, and have provided a crucial alternative to traditional confocal or bright field techniques for thin sections. However, clearing and labeling human brain tissue through all cortical layers and significant portions of a cortical area, has so far remained extremely challenging, especially for formalin fixed adult cortical tissue. Here, we present MASH (Multiscale Architectonic Staining of Human cortex): a simple, fast and low-cost cytoarchitectonic labeling approach for optically cleared human cortex samples, which can be applied to large (up to 5 mm thick) formalin fixed adult brain samples. A suite of small-molecule fluorescent nuclear and cytoplasmic dye protocols in combination with new refractive index matching solutions allows deep volume imaging. This greatly reduces time and cost of imaging cytoarchitecture in thick samples and enables classification of cytoarchitectonic layers over the full cortical depth. We demonstrate application of MASH to large archival samples of human visual areas, characterizing cortical architecture in 3D from the scale of cortical areas to that of single cells. In combination with scalable light sheet imaging and data analysis, MASH could open the door to investigation of large human cortical systems at cellular resolution and in the context of their complex 3-dimensional geometry. |
| Databáze: | OpenAIRE |
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