Production of hollow-type spherical bacterial cellulose as a controlled release device by newly designed floating cultivation
| Autor: | Kazuyoshi Yamazaki, Toru Hoshi, Takao Aoyagi, Yuki Sato, Takaya Shida |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Materials science
Gluconacetobacter xylinus Shell (structure) 02 engineering and technology 010402 general chemistry Fibril 01 natural sciences Article chemistry.chemical_compound Natural product chemistry Cellulose lcsh:Social sciences (General) lcsh:Science (General) Multidisciplinary 021001 nanoscience & nanotechnology Controlled release Silicone oil 0104 chemical sciences Membrane chemistry Chemical engineering Bacterial cellulose lcsh:H1-99 Materials chemistry 0210 nano-technology Pharmaceutical chemistry lcsh:Q1-390 |
| Zdroj: | Heliyon Heliyon, Vol 4, Iss 10, Pp e00873-(2018) |
| ISSN: | 2405-8440 |
| Popis: | We developed a novel cultivating system for hollow-type spherical bacterial cellulose (HSBC) gel production without any molds or template. It consisted of floating aqueous medium droplet containing Gluconacetobacter xylinus (G. xylinus) at the boundary of two non-mixed silicone oil layers. The fibrils of bacterial cellulose (BC) were produced at the interface of water and oil phases. Fibril layers effectively thickened layer-by-layer and eventually formed a shell structure. The size of the HSBC gel can be controlled by the volume of dropped cell suspension. For cell suspensions of 50 μL and 10 μL, HSBC gels of approximately 4.0 mm and 2.5 mm were obtained, respectively. The shell of the HSBC gel is the gelatinous membrane formed by well-organized fibril networks; they comprised type-I crystal structure of cellulose. Additionally, we studied release profile of the fluorescein isothiocyanate-dextran (FITC-Dex) and observed that it released rapidly from the HSBC gels compared to from the BC gels obtained by the static culture method. The release behavior from HSBC gel agreed satisfactorily with Higuchi model. Therefore, the shell of HSBC gel is surely a thin gelatinous membrane of BC, and would be useful as a drug release device. |
| Databáze: | OpenAIRE |
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