High-Yield Resveratrol Production in Engineered Escherichia coli
| Autor: | Zachary Fowler, Chin Giaw Lim, Thomas Hueller, Mattheos A. G. Koffas, Steffen Schaffer |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Gene Expression
Resveratrol Biology medicine.disease_cause Applied Microbiology and Biotechnology law.invention chemistry.chemical_compound law Stilbenes Gene expression Escherichia coli medicine Chromatography High Pressure Liquid Plant Proteins Ecology Strain (biology) Promoter Recombinant Proteins chemistry Biochemistry Polyphenol Yield (chemistry) Recombinant DNA Acyltransferases Metabolic Networks and Pathways Food Science Biotechnology |
| Zdroj: | Applied and Environmental Microbiology. 77:3451-3460 |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.02186-10 |
| Popis: | Plant polyphenols have been the subject of several recent scientific investigations since many of the molecules in this class have been found to be highly active in the human body, with a plethora of health-promoting activities against a variety of diseases, including heart disease, diabetes, and cancer, and with even the potential to slow aging. Further development of these potent natural therapeutics hinges on the formation of robust industrial production platforms designed using specifically selected as well as engineered protein sources along with the construction of optimal expression platforms. In this work, we first report the investigation of various stilbene synthases from an array of plant species considering structure-activity relationships, their expression efficiency in microorganisms, and their ability to synthesize resveratrol. Second, we looked into the construct environment of recombinantly expressed stilbene synthases, including different promoters, construct designs, and host strains, to create an Escherichia coli strain capable of producing superior resveratrol titers sufficient for commercial usage. Further improvement of metabolic capabilities of the recombinant strain aimed at improving the intracellular malonyl-coenzyme A pool, a resveratrol precursor, resulted in a final improved titer of 2.3 g/liter resveratrol. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|