IL-1beta sensitizes intervertebral disc annulus cells to fluid-induced shear stress
| Autor: | Mari Tsuzaki, Joe T. Minchew, Michelle Elfervig, Eric Francke, Albert J. Banes |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Adult
Discitis Adolescent Stimulation Inflammation Biochemistry Calcium in biology Proinflammatory cytokine Weight-Bearing Shear stress medicine Humans Calcium Signaling Child Intervertebral Disc Molecular Biology Cells Cultured Calcium signaling Mechanical load Lumbar Vertebrae Chemistry Intervertebral disc Cell Biology Anatomy Middle Aged Cell biology Biomechanical Phenomena medicine.anatomical_structure Female Stress Mechanical medicine.symptom Rheology Interleukin-1 |
| Zdroj: | Journal of cellular biochemistry. 82(2) |
| ISSN: | 0730-2312 |
| Popis: | Chronic inflammation and altered mechanical loading are implicated as contributors to intervertebral disc degeneration. Biomechanical and biochemical factors play a role in disc degeneration but have received limited study. Mechanically, intervertebral discs are sheared during bending or twisting of the trunk. Biochemically, IL-1beta, detected in degenerative discs, promotes metalloproteinase expression. We hypothesized that disc cells might respond to shear stress and IL-1beta in a calcium signaling response. We measured the effect of single and combined stimuli on intracellular calcium concentration ([Ca2+]ic) and signaling. Cells were isolated from annulus tissue, cultured to quiescence, plated on collagen-bonded Culture Slips and incubated with Fura-2AM. Cells then were incubated in IL-1beta. Cell response to the effects of fluid flow was tested using FlexFlo, a laminar flow device. Human annulus (hAN) cells responded to laminar fluid flow with a one to three-fold increase in [Ca2+]ic. IL-1beta alone produced a small, transient stimulation. hAN cells pretreated with IL-1beta responded to shear with a more dramatic and sustained increase in [Ca2+]ic, six to ten-fold over basal level, when compared to shear then IL-1beta or shear and IL-1beta alone (P |
| Databáze: | OpenAIRE |
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