Cross-Reactivity between Trypanosoma Cruzi and Insect Trypanosomatids as a Basis for the Diagnosis of Chagas' Disease *
Autor: | Erney P. Camargo, Barbieri Cl, Z. Caulada, Lopes Jd |
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Rok vydání: | 1981 |
Předmět: |
Chagas disease
Insecta Trypanosoma cruzi Fluorescent Antibody Technique Immunofluorescence Antigen Agglutination Tests Virology parasitic diseases medicine Animals Humans Crithidia luciliae Chagas Disease Antigens Crithidia fasciculata medicine.diagnostic_test biology Immune Sera Complement Fixation Tests fungi Complement fixation test biology.organism_classification medicine.disease Infectious Diseases Parasitology Rabbits Trypanosomiasis |
Zdroj: | The American Journal of Tropical Medicine and Hygiene. 30:1183-1188 |
ISSN: | 1476-1645 0002-9637 |
Popis: | Immunological cross-reactivity between Trypanosoma cruzi and insect trypanosomatids was demonstrated by immunofluorescence and confirmed by complement fixation, direct agglutination and cross-absorption experiments. As antigens, the following organisms were surveyed: Crithidia deanei, Crithidia fasciculata, Crithidia luciliae, Herpetomonas samuelpessoai, Herpetomonas megaseliae, Herpetomonas muscarum muscarum, Leptomonas seymouri and Blastocrithidia culicis. Sera from patients with Chagas' disease or sera from rabbits immunized against various trypanosomatids were used as sources of antibodies. The demonstration of cross-reactivity was followed by a survey of 500 human sera (from normal persons or Chagas' disease patients) by immunofluorescence using insect trypanosomatids (H. muscarum muscarum, C. fasciculata and L. seymouri) as antigens. With H. muscarum muscarum 98.7% coincident positive results and 100% of coincident negative results were obtained. These findings may validate the use of insect trypanosomatids as an alternative source of antigen in the serodiagnosis of Chagas' disease by indirect immunofluorescence. |
Databáze: | OpenAIRE |
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