Enhanced differentiation of retinal progenitor cells using microfabricated topographical cues
| Autor: | Mark R. Steedman, Sarah L. Tao, Henry Klassen, Tejal A. Desai |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Retinal degeneration
Materials science genetic structures Surface Properties Cellular differentiation Cell Culture Techniques Biomedical Engineering Biocompatible Materials Biophysics and Biological Physics Article Retina Engineering Fluid Dynamics Mice Engineering Tissue engineering Recoverin Cell Adhesion medicine Nanotechnology Animals Progenitor cell Molecular Biology Cells Cultured Tissue Engineering Tissue Scaffolds biology Stem Cells Macular degeneration Cell Differentiation Equipment Design medicine.disease eye diseases Cell biology Equipment Failure Analysis Polycaprolactone medicine.anatomical_structure Cell culture biology.protein Microfabrication sense organs Stem cell Photoreceptor Cells Vertebrate Biomedical engineering |
| Zdroj: | Biomedical Microdevices Steedman, Mark R.; Tao, Sarah L.; Klassen, Henry; & Desai, Tejal A.(2010). Enhanced differentiation of retinal progenitor cells using microfabricated topographical cues. Biomedical Microdevices: BioMEMS and Biomedical Nanotechnology, 12(3), pp 363-369. doi: 10.1007/s10544-009-9392-7. Retrieved from: http://www.escholarship.org/uc/item/0nt1w2sg Steedman, MR; Tao, SL; Klassen, H; & Desai, TA. (2010). Enhanced differentiation of retinal progenitor cells using microfabricated topographical cues. Biomedical Microdevices, 12(3), 363-369. doi: 10.1007/s10544-009-9392-7. UC Irvine: Retrieved from: http://www.escholarship.org/uc/item/9md1d1wz |
| ISSN: | 1572-8781 1387-2176 |
| DOI: | 10.1007/s10544-009-9392-7 |
| Popis: | Due to the retina's inability to replace photoreceptors lost during retinal degeneration, significant interest has been placed in methods to implant replacement cells. Polymer scaffolds are increasingly being studied as vehicles for cellular delivery to degenerated retinas. Previously, we fabricated poly(methyl methacrylate) thin film scaffolds that increased survival and integration of implanted retinal progenitor cells (RPCs). Additionally, these scaffolds minimized the trauma and cellular response associated with implantation of foreign bodies into mouse eyes. Here, we demonstrate that biodegradable polycaprolactone (PCL) thin film scaffolds can be fabricated with integrated microtopography. Microfabricated topography in a PCL thin film enhanced the attachment and organization of RPCs compared to unstructured surfaces. Using real-time quantitative polymerase chain reaction we also observed that attachment to microtopography induced cellular differentiation. RPCs grown on PCL thin films exhibited an increase in gene expression for the photoreceptor markers recoverin and rhodopsin, an increase in the glial and Müller cell marker GFAP, and a decrease in SOX2 gene expression (a marker for undifferentiated progenitor cells) compared to cells grown on unmodified tissue culture polystyrene (TCPS). © 2010 The Author(s). |
| Databáze: | OpenAIRE |
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