Species differences between rat and rabbit renal Na+/H+ exchangers
| Autor: | Gerhard Burckhardt, Jutta Sablotni, Thomas Friedrich |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
Sodium-Hydrogen Exchangers
Antiporter Biophysics Rat kidney Biology Kidney Biochemistry Amiloride Species Specificity medicine Animals Potency Molecular Biology Microvilli Vesicle Rabbit (nuclear engineering) Cell Biology Molecular biology Rats Cortex (botany) Kinetics Membrane Dicyclohexylcarbodiimide Rabbits Carrier Proteins medicine.drug |
| Zdroj: | Biochemical and Biophysical Research Communications. 144:869-875 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/s0006-291x(87)80045-1 |
| Popis: | Amiloride, 5-ethylisopropylamiloride (EIPA), and 5-ethylisopropyl-6-bromo-amiloride (Br-EIPA) inhibit rabbit and rat renal Na + /H + exchangers with comparable potency. Irreversible inhibitions by Br-EIPA after irradiation of rat and rabbit renal brush-border vesicles are also similar. In contrast, irreversible inhibition by N,N′-dicyclohexylcarbodiimide (DCCD) of the rabbit renal antiporter requires higher DCCD concentrations as compared to the rat. Rabbit renal brush-border membranes show highest [ 14 C]DCCD incorporation at MW 80,000, 51,000 and 36,000 and lack the amiloride-protectable MW 65,000 protein previously identified as Na + /H + exchanger in rat kidney cortex. The data indicate species differences with respect to renal Na + /H + exchangers. |
| Databáze: | OpenAIRE |
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