Physiological function and inflamed-brain migration of mouse monocyte-derived macrophages following cellular uptake of superparamagnetic iron oxide nanoparticles-Implication of macrophage-based drug delivery into the central nervous system
| Autor: | Yingli Shi, Hsin-I Tong, Yuanan Lu, Wen Kang, Guangzhou Zhou |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Lipopolysaccharides Male Polymers Cellular differentiation Central nervous system Pharmaceutical Science Inflammation Oleic Acids Ferric Compounds Monocytes 03 medical and health sciences Mice 0302 clinical medicine Drug Delivery Systems In vivo Medicine Cytotoxic T cell Animals Tissue Distribution Particle Size Magnetite Nanoparticles Neuroinflammation business.industry Macrophages Brain Cell Differentiation Cell biology Mice Inbred C57BL Disease Models Animal 030104 developmental biology medicine.anatomical_structure Immunology Drug delivery Acute Disease medicine.symptom business 030217 neurology & neurosurgery Homing (hematopoietic) |
| Zdroj: | International journal of pharmaceutics. 505(1-2) |
| ISSN: | 1873-3476 |
| Popis: | This study was designed to use superparamagnetic iron oxide nanoparticles (SPIONs) as evaluating tools to study monocyte-derived macrophages (MDM)-mediated delivery of small molecular agents into the diseased brains. MDM were tested with different-configured SPIONs at selected concentrations for their impacts on carrier cells' physiological and migratory properties, which were found to depend largely on particle size, coating, and treatment concentrations. SHP30, a SPION of 30-nm core size with oleic acids plus amphiphilic polymer coating, was identified to have high cellular uptake efficiency and cause little cytotoxic effects on MDM. At lower incubation dose (25 mu g/mL), few alteration was observed in carrier cells' physiological and in vivo migratory functions, as tested in a lipopolysaccharide-induced acute neuroinflammation mouse model. Nevertheless, significant increase in monocyte-to-macrophage differentiation, and decrease in in vivo carrier MDM inflamed-brain homing ability were found in groups treated with a higher dose of SHP30 at 100 mu g/mL. Overall, our results have identified MDM treatment at 25 mu g/mL SHP30 resulted in little functional changes, provided valuable parameters for using SPIONs as evaluating tools to study MDM-mediated therapeutics carriage and delivery, and supported the concepts of using monocytes-macrophages as cellular vehicles to transport small molecular agents to the brain. (C) 2016 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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