Validation of a radioimmunoassay of serum trypsin-like immunoreactivity in ferrets
| Autor: | Cory S. Bridges, John F. Engelhardt, Jan S. Suchodolski, Yaling Yi, Jonathan A. Lidbury, Pamela S. Miller, Jörg M. Steiner |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
040301 veterinary sciences Trypsinogen Radioimmunoassay Sensitivity and Specificity 0403 veterinary science Trypsin like enzyme 03 medical and health sciences chemistry.chemical_compound Species Specificity Reference Values medicine Animals Trypsin Full Scientific Reports Pancreas Reproducibility CATS Chromatography General Veterinary Chemistry Ferrets Reproducibility of Results 04 agricultural and veterinary sciences Serum samples Confidence interval Disease Models Animal 030104 developmental biology Exocrine Pancreatic Insufficiency Rabbits medicine.drug |
| Popis: | Measurement of serum trypsin-like immunoreactivity (TLI) is used to assess exocrine pancreatic function in dogs and cats. Ferrets ( Mustela putorius furo) serve as valuable animal models for human diseases such as cystic fibrosis and other pulmonary diseases, and may be a useful model of other diseases including pancreatitis. We developed and analytically validated a competitive radioimmunoassay (RIA) for measurement of TLI in ferret serum by determination of analytical sensitivity, assay linearity, accuracy of spiking recovery, precision, and reproducibility. Analytical sensitivity of the assay was 0.55 μg/L. Observed-to-expected (O/E) ratio for dilutional parallelism was 90.2–127.9% (mean: 108.1 ± 11.9%). The O/E ratio for spiking recovery was 94.5–113.0% (mean: 103.9 ± 7.2%). The intra- and inter-assay coefficients of variation (CVs) were 2.7–5.7% and 3.5–8.2%, respectively. The reference interval (RI) for serum TLI derived from 31 healthy ferrets was 28–115 μg/L; the 90% confidence interval for the lower and upper limits of the RI were 10.0–32.1 μg/L and 103–126 μg/L, respectively. This TLI RIA is analytically sensitive, sufficiently linear, accurate, precise, and reproducible for the measurement of TLI in ferret serum samples. |
| Databáze: | OpenAIRE |
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