Bacterial bioluminescence assay for bioanalysis and bioimaging
| Autor: | Xinyu He, Weinan Zhu, Yaohua Li, Haoran Li, Wei Wang |
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| Rok vydání: | 2021 |
| Předmět: |
Bioanalysis
Bacteria Chemistry Luminescent bacteria Gene Expression Regulation Bacterial Bacterial Physiological Phenomena Biochemistry Analytical Chemistry law.invention Anti-Bacterial Agents law Luminescent Measurements Recombinant DNA Bioluminescence imaging Bioluminescence Luciferase Viability assay Biosensor |
| Zdroj: | Analytical and bioanalytical chemistry. 414(1) |
| ISSN: | 1618-2650 |
| Popis: | Bioluminescence occurs through a chemical reaction in organisms that spontaneously produce light. Luminescent bacteria are unique among bioluminescent organisms. Their bioluminescence intensity is an indicator of their metabolic activity, which can directly reflect the influence of environmental factors on cell viability. Moreover, the whole bioluminescence process is totally gene encoded without the addition of extra substrates. As a result, bacterial bioluminescence has been a powerful tool for whole-cell biosensors and bio-reporters in bioanalysis and bioimaging. This review aims to cover the applications of wild-type and recombinant luminescent bacteria to detect the toxicity of environmental pollutants and biological molecules. The bacterial bioluminescence analytical assay has characteristics such as high sensitivity, short-term detection, and easy operation. Meanwhile, due to the development of gene engineering and optical technology, bacterial luciferase as a reporter protein has been successfully expressed in prokaryotic and eukaryotic cells, tissues, and organs of animals. The major applications for bacterial luciferase-based bioluminescence imaging, such as infectious diseases, cancer therapy, and stem cell tracing, are discussed in this review. |
| Databáze: | OpenAIRE |
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