The Effect of Dietary Restriction on Mitochondrial Protein Density and Flight Muscle Mitochondrial Morphology in Drosophila
| Autor: | Steve Goodall, Linda Partridge, Martin D. Brand, William B. Mair, Tapiwanashe Magwere, Jeremy Skepper |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Aging
Mitochondrion Mitochondrial Proteins In vivo medicine Animals Muscle Skeletal Drosophila Mitochondrial protein Caloric Restriction chemistry.chemical_classification Reactive oxygen species biology Life span fungi Age Factors Skeletal muscle biology.organism_classification Mitochondria Muscle Microscopy Electron Drosophila melanogaster medicine.anatomical_structure chemistry Biochemistry Geriatrics and Gerontology |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1758-535X 1079-5006 |
| Popis: | Dietary restriction (DR) extends life span in diverse organisms and may do so by attenuating production of mitochondrial reactive oxygen species (ROS). However, measurements of ROS production from isolated mitochondria of organisms subjected to DR have produced inconsistent results. In the fruit fly Drosophila, DR does not reduce production of ROS from isolated mitochondria. In this study, we used Drosophila to test whether DR lowered mitochondrial density. We assessed mitochondrial densities of flies on DR and Control diets using (a) the activities of mitochondrial enzymes and (b) electron microscopy. Both methods showed no overall effect of DR on mitochondrial density; however, mitochondrial enzyme activities and morphology differed significantly between DR and Control flies. We concluded that life-span extension by DR in Drosophila is not mediated through a reduction in mitochondrial density. If DR in Drosophila extends life span by reducing ROS production, then it does so through mechanisms that operate only in vivo. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|