Inhibition of gastric cancer cell proliferation by antisense oligonucleotides targeting the messenger RNA encoding proliferating cell nuclear antigen
| Autor: | K Ozaki, Hiroyuki Tsujimoto, M Ogaki, Oyama T, T Sakakibara, Hagiwara A, Toshio Takahashi, Chouhei Sakakura |
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| Jazyk: | angličtina |
| Rok vydání: | 1994 |
| Předmět: |
Cancer Research
Cell division Molecular Sequence Data Biology In Vitro Techniques DNA polymerase delta Stomach Neoplasms Proliferating Cell Nuclear Antigen Cell Adhesion Tumor Cells Cultured Humans RNA Messenger Nuclear protein Base Sequence Oligonucleotide Cell growth Oligonucleotides Antisense Molecular biology Proliferating cell nuclear antigen Antisense RNA Gene Expression Regulation Neoplastic Oncology Cancer cell biology.protein Cell Division Research Article |
| Zdroj: | British Journal of Cancer |
| ISSN: | 1532-1827 0007-0920 |
| Popis: | Proliferating cell nuclear antigen (PCNA) is a nuclear protein that regulates DNA synthesis by DNA polymerase delta, and is essential for DNA replication. PCNA expression level is related to the malignancy of gastric cancer cells. Seven different gastric cancer cell lines and two kinds of control cell lines were treated with antisense oligonucleotides complementary to the messenger RNA of PCNA. Treatment of each gastric cancer cell line with antisense oligonucleotides at concentration of 10-40 microM inhibited the cell growth, colony formation and PCNA protein production in a dose-dependent manner, but only affected normal cells slightly. A random sequence oligomer showed no effect. These results show that PCNA is essential for gastric cancer cell proliferation and that the use of synthetic oligonucleotides is an effective way of producing antisense-mediated changes in the behaviour of human gastric cancers. Images Figure 2 Figure 5 Figure 6 |
| Databáze: | OpenAIRE |
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