Extracellular vesicles with altered tetraspanin CD9 and CD151 levels confer increased prostate cell motility and invasion

Autor:	Martin R. Larsen, Joshua S. Brzozowski, Kathryn A. Skelding, Matthew D. Dun, Rachel Burchell, Danielle R. Bond, Helen Jankowski, Christopher J. Scarlett, Nathan D. Smith, Crystal Naudin, Belinda J. Goldie, Judith Weidenhofer
Jazyk:	angličtina
Rok vydání:	2018
Předmět:	0301 basic medicine Male Proteome Population Motility lcsh:Medicine Biology Protein degradation Tetraspanin 24 Tetraspanin 29 Metastasis Cell Line 03 medical and health sciences Prostate cancer Extracellular Vesicles Tetraspanin Prostate Cell Movement medicine Humans education lcsh:Science education.field_of_study Multidisciplinary lcsh:R Cell migration medicine.disease Cell biology 030104 developmental biology medicine.anatomical_structure embryonic structures lcsh:Q
Zdroj:	Scientific Reports, Vol 8, Iss 1, Pp 1-13 (2018) Brzozowski, J S, Bond, D R, Jankowski, H, Goldie, B J, Burchell, R, Naudin, C, Smith, N D, Scarlett, C J, Larsen, M R, Dun, M D, Skelding, K A & Weidenhofer, J 2018, ' Extracellular vesicles with altered tetraspanin CD9 and CD151 levels confer increased prostate cell motility and invasion ', Scientific Reports, vol. 8, 8822 . https://doi.org/10.1038/s41598-018-27180-z
ISSN:	2045-2322
DOI:	10.1038/s41598-018-27180-z
Popis:	To facilitate intercellular communication, cells release nano-sized, extracellular vesicles (EVs) to transfer biological cargo to both local and distant sites. EVs are enriched in tetraspanins, two of which (CD9 and CD151) have altered expression patterns in many solid tumours, including prostate cancer, as they advance toward metastasis. We aimed to determine whether EVs from prostate cells with altered CD9 and CD151 expression could influence cellular behaviour and increase the metastatic capabilities of non-tumourigenic prostate cells. EVs were isolated by ultrafiltration and characterised for their tetraspanin expression and size distribution. iTRAQ was used to identify differences between RWPE1 and tetraspanin-modified RWPE1 EV proteomes, showing an enrichment in protein degradation pathways. Addition of EVs from RWPE1 cells with reduced CD9 or increased CD151 abundance resulted in increased invasion of RWPE1 cells, and increased migration in the case of high CD151 abundance. We have been able to show that alteration of CD9 and CD151 on prostate cells alters the proteome of their resultant EVs, and that these EVs can enhance the migratory and invasive capabilities of a non-tumourigenic prostate cellular population. This work suggests that cellular tetraspanin levels can alter EVs, potentially acting as a driver of metastasis in prostate cancer.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::975da3d934a35a8bb2d1f0981ed8842a http://link.springer.com/article/10.1038/s41598-018-27180-z Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
Nepřihlášeným uživatelům se plný text nezobrazuje	K zobrazení výsledku je třeba se přihlásit.