Human placental mesenchymal stem cells regulate inflammation via the NF‑κB signaling pathway
| Autor: | Youyi, Liu, Xiading, Zhang, Yiwei, Hu, Mingzhu, Kang, Yibo, Wu, Yuan, Wang, Chao, Deng |
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| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Experimental and Therapeutic Medicine. 24 |
| ISSN: | 1792-1015 1792-0981 |
| DOI: | 10.3892/etm.2022.11591 |
| Popis: | Emerging evidence has indicated that mesenchymal stem cells (MSCs) are involved in the modulation of inflammation. Human placenta-derived (HPL)-MSCs exist in sufficient quantities and play a role in immune regulation. However, the exact roles of HPL-MSCs in inflammation and the specific underlying mechanisms are not well defined. In the present study, HPL-MSCs were obtained from human fetal placentas, and further purified using a commercial kit. Using ELISA, reverse transcription-quantitative PCR, western blot, NO detection and other assays, the present study revealed that HPL-MSCs may improve lipopolysaccharide-induced macrophage inflammation by regulating macrophage polarization. Further mechanistic studies demonstrated that HPL-MSCs attenuated the NF-κB signaling pathway by regulating the expression of toll-like receptor 4 and the phosphorylation of IκBα and p65, which resulted in a reduction in the levels of inflammation. The present study indicated that HPL-MSCs may act as a novel target for the treatment of inflammation-related diseases. |
| Databáze: | OpenAIRE |
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