Penaeus monodon tropomyosin induces CD4 T-cell proliferation in shrimp-allergic patients
| Autor: | Matthew F. Cusick, Ann Georgelas, David D. Eckels, Igor Y. Pavlov, Eugene V. Ravkov, Shuping Wang, Gerald J. Gleich, Julio C. Delgado, Kate Sebastian, Lori A. Wagner |
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| Rok vydání: | 2012 |
| Předmět: |
CD4-Positive T-Lymphocytes
Allergy animal structures Molecular Sequence Data Immunology Population Tropomyosin macromolecular substances Lymphocyte Activation Immunoglobulin E Peripheral blood mononuclear cell Article Cell Line Penaeus monodon Penaeidae medicine Animals Humans Immunology and Allergy Amino Acid Sequence education Skin Tests education.field_of_study biology fungi Shellfish allergy General Medicine Allergens biology.organism_classification medicine.disease Shrimp biology.protein Peptides Food Hypersensitivity |
| Zdroj: | Human Immunology. 73:426-431 |
| ISSN: | 0198-8859 |
| DOI: | 10.1016/j.humimm.2011.12.019 |
| Popis: | Shellfish allergy affects approximately 2% of the population and can cause immediate hypersensitivity reactions such as urticaria, swelling, difficulty breathing, and, in some cases, anaphylaxis. Tropomyosin is the major shrimp allergen and binds IgE in two-thirds of patients. A total of 38 shrimp-allergic patients and 20 negative control subjects were recruited and evaluated on the basis of history, skin prick testing, specific immunoglobulin E (IgE) levels, and peripheral blood mononuclear cell proliferation in response to shrimp tropomyosin or shrimp tropomyosin-derived peptides. Of the classically allergic patients by history, 59% tested positive for serum shrimp IgE antibodies. Of patients with shrimp-specific IgE in sera, 70% also had significant IgE levels specific for shrimp tropomyosin. Peripheral blood mononuclear cells from classically shrimp-allergic patients proliferated in a dose-dependent manner in response to to tropomyosin. In addition, a T-cell line derived from a shrimp-allergic patient proliferated specifically in response to tropomyosin-derived peptides. These studies suggest a strategy for immunotherapy using a tropomyosin-derived T-cell epitope vaccination. |
| Databáze: | OpenAIRE |
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