Demonstration of an ecdysteroid receptor in a cultured cell line of Drosophila melanogaster
| Autor: | Christoph Beckers, Roger Dennis, Becky A. Sage, Péter Maróy, John D. O'Connor |
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| Rok vydání: | 1978 |
| Předmět: |
Cell Nucleus
Cytoplasm Ecdysone Multidisciplinary biology Receptors Cell Surface biology.organism_classification Ligand (biochemistry) Molecular biology Binding Competitive Cell Line Dissociation constant Cytosol Cell nucleus medicine.anatomical_structure Drosophila melanogaster Ecdysterone Cell culture Insect Hormones medicine Receptor Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 75(12) |
| ISSN: | 0027-8424 |
| Popis: | Incubation of the high-speed supernatant from the Kc cell line of Drosophila melanogaster with [3H]ponasterone A results in significant binding of the ligand as determined by gel filtration and dextran-coated charcoal binding assays. In vivo exposure of the Kc line to [3H]ponasterone A for 30 min results in a marked binding of the ligand by a KCl-soluble nuclear extract. With both the cytosol and nuclear preparations the binding has specificity and a low dissociation constant (3 X 10(-9) M). In addition the labeling of the nuclear preparation exhibits a saturation at approximately 7 X 10(-10) M which probably reflects the molar concentration of cytoplasmic receptors. |
| Databáze: | OpenAIRE |
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