Transcription antitermination in vitro by lambda N gene product: requirement for a phage nut site and the products of host nusA, nusB, and nusE genes
| Autor: | Asis Das, Krystyna Wolska |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Genetics
biology Genes Viral Transcription Genetic Mutant Genetic Complementation Test DNA Restriction Enzymes Templates Genetic Lambda phage biology.organism_classification Bacteriophage lambda General Biochemistry Genetics and Molecular Biology Complementation Transcription antitermination Galactokinase Viral Proteins Plasmid Cistron Genes Genes Bacterial Antitermination Escherichia coli Gene Ribosomes Plasmids |
| Zdroj: | Cell. 38(1) |
| ISSN: | 0092-8674 |
| Popis: | Employing specifically engineered plasmids in which the expression of E. coli galK cistron is regulated by transcription termination, we have analyzed the antitermination function of phage lambda N gene product in S30 extracts. Antitermination by N, dependent on its site of action, nutL, is defective in the extracts prepared from nusA, nusB, and nusE mutants. By complementation analysis, we demonstrate that none of the these nus mutations affects the synthesis of N or the other nus gene products to cause a defect in antitermination. Rather, these mutations have inactivated a set of specific host components, the Nus factors, which are essential for N activity. Curiously, an appreciable portion of N and Nus complementation activities of an S30 extract is ribosome-associated. The significance of this finding remains to be uncovered. |
| Databáze: | OpenAIRE |
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