Methodology for biomechanical testing of fresh anterior wall vaginal samples from postmenopausal women undergoing cystocele repair
| Autor: | Aradhana Bhatt, Philippe E. Zimmern, Robert C. Eberhart |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Urology Anterior wall Uniaxial tension Dentistry In Vitro Techniques Biomechanical testing Cystocele repair Specimen Handling Uterine Prolapse Tensile Strength Ultimate tensile strength Tissue hydration medicine Humans Aged Postmenopausal women business.industry Biomechanics Water Middle Aged Biomechanical Phenomena Surgery Postmenopause Vagina Female Stress Mechanical Neurology (clinical) business Cystocele |
| Zdroj: | Neurourology and Urodynamics. 28:325-329 |
| ISSN: | 1520-6777 0733-2467 |
| Popis: | Goal To explore the methodological challenges of biomechanical testing of freshly harvested human anterior vaginal wall (HAVW) samples. Method Longitudinal full-thickness samples of HAVW were excised during cystocele repair in postmenopausal women and age-matched controls. Two methods of tissue storage during transport were compared. All samples were prepared for uniaxial testing within 2 hr of harvest and loaded at a rate of 0.5 mm/sec, until irreversible deformation was observed. Young's modulus and other parameters were extracted from the tensile stress–strain curves. Results Samples were obtained over 2 years from 42 patients. Significant differences in biomechanical parameters were noted based on the degree of hydration of the tissue, suggesting that the wetter samples were mechanically weaker. Conclusions This study reports on a new method for testing the biomechanical properties of freshly harvested HAVW tissues and the impact of tissue hydration during transport between the operating room and the testing lab. Neurourol. Urodynam. 28:325–329, 2009. © 2009 Wiley-Liss, Inc. |
| Databáze: | OpenAIRE |
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