Paneth Cells in Intestinal Homeostasis and Tissue Injury
| Autor: | Owen J. Sansom, Riccardo Fodde, Sabrina Roth, Andrea Sacchetti, Andreas Kremer, Patrick Franken, Kurt I. Anderson |
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| Přispěvatelé: | Pathology |
| Rok vydání: | 2012 |
| Předmět: |
Male
Mouse Cellular differentiation lcsh:Medicine Fluorescent Antibody Technique Enteroendocrine cell Polymerase Chain Reaction Receptors G-Protein-Coupled Mice Molecular Cell Biology Intestine Small Stem Cell Niche Intestinal Mucosa lcsh:Science Cells Cultured Oligonucleotide Array Sequence Analysis Polycomb Repressive Complex 1 Multidisciplinary Stem Cells Cell Cycle LGR5 Animal Models Cell cycle Flow Cytometry Immunohistochemistry Cell biology Intestines Adult Stem Cells medicine.anatomical_structure Medicine Small Intestine Female Stem cell Cellular Types Adult stem cell Research Article Paneth Cells Enterocyte Mice Transgenic Gastroenterology and Hepatology Biology digestive system Model Organisms Proto-Oncogene Proteins medicine Animals Cell Proliferation lcsh:R Epithelial Cells Paneth cell lcsh:Q Developmental Biology |
| Zdroj: | PLoS ONE PLoS One (print), 7(6). Public Library of Science PLoS ONE, Vol 7, Iss 6, p e38965 (2012) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0038965 |
| Popis: | Adult stem cell niches are often co-inhabited by cycling and quiescent stem cells. In the intestine, lineage tracing has identified Lgr5(+) cells as frequently cycling stem cells, whereas Bmi1(+), mTert(+), Hopx(+) and Lrig1(+) cells appear to be more quiescent. Here, we have applied a non-mutagenic and cell cycle independent approach to isolate and characterize small intestinal label-retaining cells (LRCs) persisting in the lower third of the crypt of Lieberkühn for up to 100 days. LRCs do not express markers of proliferation and of enterocyte, goblet or enteroendocrine differentiation, but are positive for Paneth cell markers. While during homeostasis, LR/Paneth cells appear to play a supportive role for Lgr5(+) stem cells as previously shown, upon tissue injury they switch to a proliferating state and in the process activate Bmi1 expression while silencing Paneth-specific genes. Hence, they are likely to contribute to the regenerative process following tissue insults such as chronic inflammation. |
| Databáze: | OpenAIRE |
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