A rapid procedure for bacterial identification and antimicrobial susceptibility testing directly from positive blood cultures
| Autor: | Chen Chen, Pu Wang, Xixiong Kang, Weifeng Zhang, Li Rui, Bo Sun, Yubo Fan, Shipei He, Shuhua Yue, Lu Lan, Weili Hong, Guang-Hui Zheng, Chenxi Zhang |
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| Rok vydání: | 2022 |
| Předmět: |
Chromatography
Desorption ionization biology Chemistry Antimicrobial susceptibility Mass spectrometry biology.organism_classification Biochemistry Analytical Chemistry Anti-Infective Agents Blood Culture Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Positive blood culture Electrochemistry Environmental Chemistry Spectroscopy Bacteria |
| Zdroj: | The Analyst. 147:147-154 |
| ISSN: | 1364-5528 0003-2654 |
| DOI: | 10.1039/d1an01210j |
| Popis: | There is an urgent need to develop a rapid procedure that can rapidly identify and obtain antimicrobial susceptibility testing (AST) results directly from positive blood cultures. Here, we report a semi-automatic bacterial diagnosis procedure, which includes 1) a bacterial concentration process to isolate the bacteria from a positive blood culture bottle (PBCB), 2) an identification process using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), and 3) a rapid AST process based on stimulated Raman scattering imaging of deuterium oxide (D2O) incorporation in bacteria. A total of 105 samples were tested for bacterial identification, and bacterial identification accuracy of 92.3% was achieved. AST takes about 2.5 h after identification. This semi-automatic procedure only takes 3.5 h, which is demonstrated to be the fastest process to obtain identification and AST results starting from PBCB. |
| Databáze: | OpenAIRE |
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