Transforming Growth Factor-α and Epidermal Growth Factor Receptor in Colonic Mucosa in Active and Inactive Inflammatory Bowel Disease
| Autor: | Thérèse Lehy, Dessirier, M. J. M. Lewin, Khadija Hormi, Guillaume Cadiot, Michel Mignon, Le Romancer M, Stéphanie Kermorgant |
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| Rok vydání: | 2000 |
| Předmět: |
Adult
Male TGF alpha Pathology medicine.medical_specialty Colon Biopsy Blotting Western Clinical Biochemistry Inflammatory bowel disease Endocrinology Crohn Disease Humans Medicine Tissue Distribution Epidermal growth factor receptor Intestinal Mucosa Colitis Receptor Aged Aged 80 and over Crohn's disease biology business.industry Colonoscopy Cell Biology Middle Aged Transforming Growth Factor alpha Inflammatory Bowel Diseases medicine.disease Immunohistochemistry Ulcerative colitis digestive system diseases ErbB Receptors biology.protein Colitis Ulcerative Female business |
| Zdroj: | Growth Factors. 18:79-91 |
| ISSN: | 1029-2292 0897-7194 |
| DOI: | 10.3109/08977190009003235 |
| Popis: | Transforming growth factor-alpha (TGF-alpha) is overexpressed in colonic carcinomas and promotes mucosal wound healing. It may be implicated in chronic inflammatory bowel disease (IBD). We analyzed the expression of TGF-alpha and its receptor, epidermal growth factor receptor (EGF-r), in the colonic mucosa of patients with Crohn's disease (CD) or ulcerative colitis (UC), in active or inactive stages, as compared with controls. Proteins and mRNA were detected in biopsies from the right and left colon and in surgical colonic specimens. Immunoblot analysis revealed TGF-alpha protein as a 29 kDa band. This band was normally expressed in uninvolved colonic mucosa of patients with CD or UC whether in active or inactive stages, but decreased or absent in involved mucosa of active IBD, even when TGF-alpha mRNA and EGF-r protein were detected. In the unaffected mucosa of CD, the intensity of TGF-alpha immunoreactivity was similar to that of controls in the right colon but stronger (P = 0.05) in the left colon. There was no TGF-alpha overexpression in dysplastic regions. In conclusion, in active IBD disease, the decreased TGF-alpha protein amount seems not only related to epithelial cell loss but reflects a down-regulation at least at the protein level. We speculate that TGF-alpha does not play a role within the active stage but may be implicated later in the repair process. |
| Databáze: | OpenAIRE |
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