Phosphorylation-dependent control of an RNA granule-localized protein that fine-tunes defence gene expression at a post-transcriptional level
| Autor: | Lennart Eschen-Lippold, Martina Brode, Justin Lee, Benedikt Athmer, Wolfgang Hoehenwarter, Manaswita Baruah, Dierk Scheel, Naheed Tabassum, Gerd Hause, Luis David Maldonado-Bonilla |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Arabidopsis Gene Expression Plant Science Biology 01 natural sciences Transcriptome 03 medical and health sciences Stress granule Gene Expression Regulation Plant Gene expression Genetics Plant Immunity RNA Messenger Phosphorylation Protein kinase A Post-transcriptional regulation Plant Diseases Messenger RNA Protein turnover RNA-Binding Proteins Translation (biology) Cell Biology Cell biology Protein Transport 030104 developmental biology 010606 plant biology & botany Signal Transduction Transcription Factors |
| Zdroj: | The Plant journal : for cell and molecular biologyReferences. 101(5) |
| ISSN: | 1365-313X |
| Popis: | Mitogen-activated protein kinase (MAPK) cascades are key signalling modules of plant defence responses to pathogen-associated molecular patterns [PAMPs; e.g. the bacterial peptide flagellin (flg22)]. Tandem zinc finger protein 9 (TZF9) is a RNA-binding protein that is phosphorylated by two PAMP-responsive MAPKs, MPK3 and MPK6. We mapped the major phosphosites in TZF9 and showed their importance for controlling in vitro RNA-binding activity, in vivo flg22-induced rapid disappearance of TZF9-labelled processing body-like structures and TZF9 protein turnover. Microarray analysis showed a strong discordance between transcriptome (total mRNA) and translatome (polysome-associated mRNA) in the tzf9 mutant, with more mRNAs associated with ribosomes in the absence of TZF9. This suggests that TZF9 may sequester and inhibit the translation of subsets of mRNAs. Fittingly, TZF9 physically interacts with poly(A)-binding protein 2 (PAB2), a hallmark constituent of stress granules - sites for stress-induced translational stalling/arrest. TZF9 even promotes the assembly of stress granules in the absence of stress. Hence, MAPKs may control defence gene expression post-transcriptionally through release from translation arrest within TZF9-PAB2-containing RNA granules or by perturbing the function of PAB2 in translation control (e.g. in the mRNA closed-loop model of translation). |
| Databáze: | OpenAIRE |
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