Effect of IRT5 probiotics on dry eye in the experimental dry eye mouse model

Autor: Sin-Hyeog Im, Jayoon Moon, Mee Kum Kim, Jun Yeop Kim, Jin Suk Ryu
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Male
0301 basic medicine
Physiology
medicine.medical_treatment
Administration
Oral
Cornea
Mice
0302 clinical medicine
RNA
Ribosomal
16S
Lactobacillus
Medicine and Health Sciences
Tear secretion
Saline
Multidisciplinary
biology
Genomics
Animal Models
medicine.anatomical_structure
Experimental Organism Systems
Medical Microbiology
Medicine
Dry Eye Syndromes
Goblet Cells
Anatomy
Conjunctiva
Research Article
medicine.medical_specialty
Ocular Anatomy
Science
Alpha (ethology)
Mouse Models
Microbial Genomics
Lacrimal gland
Research and Analysis Methods
Real-Time Polymerase Chain Reaction
Microbiology
03 medical and health sciences
Model Organisms
Ocular System
Internal medicine
Genetics
medicine
Animals
Secretion
Goblet cell
Bacteria
Tumor Necrosis Factor-alpha
Probiotics
Gut Bacteria
Organisms
Biology and Life Sciences
biology.organism_classification
Gastrointestinal Microbiome
Gastrointestinal Tract
Mice
Inbred C57BL
Disease Models
Animal
030104 developmental biology
Endocrinology
Tears
Animal Studies
Eyes
Microbiome
Physiological Processes
Digestive System
Head
030217 neurology & neurosurgery
Zdroj: PLoS ONE, Vol 15, Iss 12, p e0243176 (2020)
PLoS ONE
ISSN: 1932-6203
Popis: Objective To investigate the clinical effects of IRT5 probiotics in the environmental dry eye model. Methods Eight week old male C57BL/6 mice were randomly divided into two groups; control group (n = 16) received oral gavage of 300 μL phosphate-buffered saline (PBS) alone once daily, IRT5 group (n = 9) received oral gavage of 1 x 109 CFU IRT5 probiotics powder in 300 μL PBS once daily, both groups for 11 to 12 days. Simultaneously, all mice underwent dry eye induction. Tear secretion, corneal staining and conjunctival goblet cell density were evaluated. Quantative real-time polymerase chain reaction (RT-PCR) for inflammation-related markers was performed. 16S ribosomal RNA of fecal microbiome was analyzed and compositional difference, alpha and beta diversities were assessed. Results There was no difference in NEI score but significant increase in tear secretion was observed in IRT5 group (p < 0.001). There was no significant difference in goblet cell density between groups. Quantative RT-PCR of cornea and conjunctiva revealed increased TNF-α expression in IRT5 group (p < 0.001) whereas other markers did not significantly differ from control. IRT5 group had significantly increased species diversity by Shannon index (p = 0.041). Beta diversity of genus by UniFrac principle coordinates analysis showed significant distance between groups (p = 0.001). Compositional differences between groups were observed and some were significantly associated with tear secretion. Multivariate linear regression analysis revealed Christensenellaceae (p = 0.009), Lactobacillus Helveticus group (p = 0.002) and PAC001797_s (p = 0.011) to strongly influence tear secretion. Conclusion In experimental dry eye model, IRT5 probiotics treatment partially improves experimental dry eye by increasing tear secretion which was associated with and influenced by the change in intestinal microbiome. Also, intestinal microbiome may affect the lacrimal gland through a different mechanism other than regulating inflammation.
Databáze: OpenAIRE
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