Cross Talk with Hematopoietic Cells Regulates the Endothelial ProgenitorCell Differentiation of CD34 Positive Cells
| Autor: | So Young Yoo, Yeon-Ju Kim, Jong-Kyu Hong, Takayuki Asahara, Ji-Hye Park, Jun Hee Lee, Seok-Yun Jung, Sung Wook Kim, Jung-Hee Kim, Songhwa Kang, Da-Yeon Kim, Sun-Jin Lee, Hwi-Gon Kim, Sang Hun Lee, Sang-Mo Kwon |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
Cell Physiology Physiology Cellular differentiation Science CD34 Antigens CD34 Biology Bioinformatics Cardiovascular Physiology Endothelial progenitor cell Mice Ischemia Human Umbilical Vein Endothelial Cells Animals Humans Progenitor cell Cells Cultured Interleukin 3 Endothelial Progenitor Cells Mice Inbred BALB C Multidisciplinary Regeneration (biology) Biology and Life Sciences Cell Differentiation Cell Biology Fetal Blood Coculture Techniques Cell biology Hindlimb Endothelial stem cell Haematopoiesis Disease Models Animal embryonic structures cardiovascular system Medicine Cord Blood Stem Cell Transplantation Research Article circulatory and respiratory physiology |
| Zdroj: | PLOS ONE(9): 8 PLoS ONE PLoS ONE, Vol 9, Iss 8, p e106310 (2014) |
| Popis: | Introduction: Despite the crucial role of endothelial progenitor cells (EPCs) in vascular regeneration, the specific interactions between EPCs and hematopoietic cells remain unclear. Methods: In EPC colony forming assays, we first demonstrated that the formation of EPC colonies was drastically increased in the coculture of CD34(+) and CD34(-) cells, and determined the optimal concentrations of CD34(+) cells and CD34(-) cells for spindle-shaped EPC differentiation. Results: Functionally, the coculture of CD34(+) and CD34(-) cells resulted in a significant enhancement of adhesion, tube formation, and migration capacity compared with culture of CD34(+) cells alone. Furthermore, blood flow recovery and capillary formation were remarkably increased by the coculture of CD34(+) and CD34(-) cells in a murine hind-limb ischemia model. To elucidate further the role of hematopoietic cells in EPC differentiation, we isolated different populations of hematopoietic cells. T lymphocytes (CD3(+)) markedly accelerated the early EPC status of CD34(+) cells, while macrophages (CD11b(+)) or megakaryocytes (CD41(+)) specifically promoted large EPC colonies. Conclusion: Our results suggest that specific populations of hematopoietic cells play a role in the EPC differentiation of CD34(+) cells, a finding that may aid in the development of a novel cell therapy strategy to overcome the quantitative and qualitative limitations of EPC therapy. |
| Databáze: | OpenAIRE |
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