Lipopolysaccharide-induced increase in plasma nitrotyrosine concentrations in rats
| Autor: | Yoshinori Kamisaki, Kumiyo Ashida, Yosuke Kishimoto, Yoshinori Yamada, Kouichirou Wada, Masayuki Ataka, Kentaro Nakamoto |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Lipopolysaccharides
Male medicine.medical_specialty Lipopolysaccharide Nitrite Oxidative phosphorylation Nitrate Peroxynitrite Sepsis chemistry.chemical_compound Internal medicine medicine Animals Rats Wistar Molecular Biology Nitrites Nitrotyrosine Nitrates medicine.disease Pathophysiology Endotoxemia Rats 4-Chloro-7-nitrobenzofurazan Endocrinology chemistry Biochemistry Tyrosine Molecular Medicine |
| Zdroj: | Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. (1):24-28 |
| ISSN: | 0925-4439 |
| DOI: | 10.1016/S0925-4439(97)00052-5 |
| Popis: | Since the production of peroxynitrite may contribute to the pathophysiology of endotoxemia or sepsis, the quantities of the produced peroxynitrite were evaluated in rats after lipopolysaccharide (LPS) treatment by measuring plasma nitrotyrosine concentrations with a new method. The intraperitoneal administration of LPS caused a persistent increase in plasma nitrotyrosine concentrations, which reached a maximum with 6-fold level of the base line (105 pmol ml −1 ) at 24 h and gradually declined to 3-fold level of the base line at 7 days. However, plasma concentrations of nitrite and nitrate peaked at 18 h, returning to base line within 48 h. The effect of LPS on the increase in plasma concentration of nitrotyrosine was dose-dependent and consistent with that of nitrite and nitrate concentrations. On the other hand, intravenous injection of nitrotyrosine revealed a rapid clearance with a plasma half-life of 1.67 h. These results indicate that the elevation of plasma nitrotyrosine concentrations may persist for more than a week after LPS treatment, and that the determination of plasma nitrotyrosine concentrations may be useful to detect the previous peroxynitrite-dependent oxidative damages. |
| Databáze: | OpenAIRE |
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