Immunoelectron microscopic studies of glycosaminoglycans in the metaphyseal bone trabeculae of growing rats
| Autor: | Minoru Takagi, Teruhiko Ishii, Toshitada Kazama, Yoshihisa Toda |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
Pathology
medicine.medical_specialty Bone Matrix Metaphysis Bone and Bones Glycosaminoglycan Immunoenzyme Techniques chemistry.chemical_compound Calcification Physiologic medicine Chondroitin Animals Growth Plate Rats Wistar Microscopy Immunoelectron Glycosaminoglycans Osteoblasts Tibia Osteoid Cartilage Antibodies Monoclonal Cell Biology medicine.disease Staining Rats medicine.anatomical_structure chemistry Bone Trabeculae Anatomy Calcification |
| Zdroj: | The Histochemical journal. 24(10) |
| ISSN: | 0018-2214 |
| Popis: | The types and distribution of glycosaminoglycans (GAGs) were studied immunocytochemically in osteoid, mineralized bone matrix, and cartilage matrix of growing rat metaphyseal bone after aldehyde fixation and EDTA demineralization, using four monoclonal antibodies (mAbs 1-B-5, 2-B-6, 3-B-3 and 5-D-4). These mAbs specifically recognize epitopes in non-sulphated chondroitin (C0-S); chondroitin 4-sulphate (C4-S) and dermatan sulphate (DS); chondroitin 6-sulphate (C6-S) and C0-S; and keratan sulphate (KS) respectively. In osteoid, all mAbs except 1-B-5 weakly stained matrix material on and between collagen fibrils, and moderately stained organic material corresponding to bone nodules, which are known sites of mineralization. However, the staining of osteoid abruptly decreased at the mineralization front; weak staining was confined mostly to the organic material of bone nodules in mineralized bone matrix, with very weak or no staining of the rest of the bone matrix. This staining progressively decreased toward the mineralized cartilage matrix and became negative. The mineralized cartilage matrix and lamina limitans reacted strongly with all mAbs except 5-D-4. These results indicate that osteoid contains sulphated proteoglycans containing C4-S and/or DS, C6-S and KS, and subsequent bone matrix mineralization appears to require accumulation of these macromolecules within bone nodules and eventual loss of these substances for complete mineralization, whereas proteoglycans containing C0-S, C4-S and/or DS, and C6-S, still exist in mineralized cartilage matrix and lamina limitants. |
| Databáze: | OpenAIRE |
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