Optimization of accessible catalase activity in polyacrylamide gel-immobilized Saccharomyces cerevisiae
| Autor: | John E. Seip, Robert Di Cosimo |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
chemistry.chemical_classification
Chromatography biology Saccharomyces cerevisiae Polyacrylamide Bioengineering biology.organism_classification Applied Microbiology and Biotechnology Yeast chemistry.chemical_compound Enzyme nervous system chemistry Biochemistry Catalase Bromide biology.protein Polyacrylamide gel electrophoresis Biotechnology |
| Zdroj: | Biotechnology and bioengineering. 40(5) |
| ISSN: | 0006-3592 |
| Popis: | The permeabilization of Saccharomyces cerevisiae (baker's yeast), either before or after immobilization in polyacrylamide gel (PAG), has been examined as a means to increase the catalase activity of PAG-immobilized yeast cells. Prior permeabilization of the cells resulted in large losses of catalase activity during immobilization, but permeabilization after immobilization produced increases in the catalase activity of yeast/PAG particles. A dependence of the accessible catalase activity on the concentration of polyacrylamide in permeabilized yeast/PAG particles, and on the method of permeabilization of the immobilized cells, was observed. Optimal levels of stable catalase activity (1000–2000 IU/g PAG particles; ca. 5%–10% of total available activity) were obtained by immobilizing yeast cells (0.5 g wet cells/mL gel) in 10% (w/v) PAG, followed by permeabilization of the entrapped cells with either cetyltrimethylammonium bromide, Triton X-100 and one freeze-thaw, or five freeze-thaw cycles. © 1992 John Wiley & Sons, Inc. |
| Databáze: | OpenAIRE |
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