Functional and nonfunctional measles virus matrix genes from lethal human brain infections
| Autor: | Anita M. Schmid, I. Ballart, Martin A. Billeter, M. Huber, Roberto Cattaneo |
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| Jazyk: | angličtina |
| Rok vydání: | 1991 |
| Předmět: |
Genes
Viral Paramyxoviridae Sequence analysis Molecular Sequence Data Immunology Recombinant virus Microbiology Subacute sclerosing panencephalitis Virus Cell Line Viral Matrix Proteins Measles virus Virology medicine Animals Humans Gene Glycoproteins SSPE Virus Base Sequence biology virus diseases medicine.disease biology.organism_classification Retraction Insect Science DNA Viral Mutation Subacute Sclerosing Panencephalitis Plasmids Research Article |
| Popis: | Subacute sclerosing panencephalitis (SSPE) is a lethal disease induced by the persistence of measles virus in the human brain. In many SSPE cases, the viral matrix (M) protein cannot be detected; in others, M proteins of the expected size are found and sequence analysis of M cDNAs has confirmed that the reading frames are intact, showing only several missense mutations. To determine whether these alterations result in nonfunctional proteins, we have replaced the M gene of an infectious full-length genomic cDNA (from vaccine strain Edmonston) with different M genes derived from four patients with SSPE. One of the SSPE M genes tested proved to be functionally competent, giving rise to a virus yielding titers similar to those of viruses containing the M gene from control lytic strains. The other three SSPE M genes were not functionally competent in the same test. In all three cases, the inactivating changes resided in the carboxyl-terminal half of the M protein, as shown by the exchange of either of the two genes halves. In summary, mutational M gene alterations, which either prevent synthesis of M protein altogether or only allow synthesis of nonfunctional M protein, have been detected by us and by others in 9 of 10 SSPE cases. The one functional M gene appears to be an exception to the rule, indicating that M gene alteration might not be an absolute requirement for disease development. |
| Databáze: | OpenAIRE |
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