Prediction of reversibility of intestinal mucosal damage after ischemia-reperfusion injury by plasma intestinal fatty acid-binding protein levels in pigs
| Autor: | Hiroya Kano, Yutaka Okita, Keisuke Morimoto, Keigo Fukase, Kenji Okada, Akihiko Ito, Wulan Bao |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty Pathology Swine Ischemia Fatty Acid-Binding Proteins Fatty acid-binding protein Immunoenzyme Techniques Intestinal mucosa Predictive Value of Tests Internal medicine Animals Medicine Radiology Nuclear Medicine and imaging Intestinal Mucosa Advanced and Specialized Nursing business.industry Cadherin Intestinal ischemia General Medicine medicine.disease Intestinal Diseases Endocrinology Reperfusion Injury Immunoenzyme techniques Intestinal Fatty Acid-Binding Protein Female Cardiology and Cardiovascular Medicine business Safety Research Reperfusion injury Biomarkers |
| Zdroj: | Perfusion. 30:617-625 |
| ISSN: | 1477-111X 0267-6591 |
| Popis: | Objective: The aims of this study were to elucidate the association between plasma intestinal fatty acid-binding protein (I-FABP) level and actual pathological damage of intestinal mucosa and its reversibility. Methods: An intestinal ischemia-reperfusion model was created by temporary occlusion of the descending aorta in 9 pigs which were divided into 3 groups according to the duration of visceral ischemic insult: 15-minute ischemia (n=3), 30-minute ischemia (n=3) and 60-minute ischemia (n=3). Blood samples and short segments of the jejunum for pathological examinations, including immunohistochemical staining of I-FABP, Ki-67 and E-cadherin, were taken at the beginning of the operation (T1) and 15 minutes (T2), 30 minutes (T3), 45 minutes (T4) and 60 minutes (T5) after reperfusion. Results: Plasma I-FABP after 15 minutes of ischemia reached a peak of 1859±1089 pg/ml at T3, while the level after 30 minutes of ischemia achieved a peak level of 5053±1717 pg/ml at T5. The level after 60 minutes of ischemia demonstrated a rapid increment up to 10734±93 pg/ml at T3. There was a significant difference in the trend of plasma I-FABP levels between 30 minutes and 60 minutes of ischemia (p=0.01). The strongest immunohistochemical staining of the intestinal epithelium for I-FABP was observed at T4 after 30 minutes of ischemia, with the shedding of injured epithelium followed by re-epithelialisation, with sequential up-regulation of Ki67 and E-cadherin. However, the intestinal epithelium after 60 minutes of ischemia demonstrated the lack of I-FABP expression with irreversible damage. Conclusion: Plasma I-FABP levels may be a crucial marker to recognize the reversibility of damage of the intestinal epithelium after an ischemic insult and the level of 5000 pg/ml is considered to be the critical borderline for irreversibility, which might prevent diagnostic delay in the clinical setting. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|