Safety and efficacy of suicide gene therapy with adenosine deaminase 5-fluorocytosine silmutaneously in in vitro cultures of melanoma and retinal cell lines
| Autor: | Wolfgang Hohenforst-Schmidt, Soultana Meditskou, Kalliopi Domvri, Ioannis Kioumis, Thomas Zarampoukas, Paul Zarogoulidis, Theodora Tsiouda, Georgia Pitsiou, Dimitris Bougiouklis, Nikolaos Pezirkianidis, Qiang Li, Konstantinos Zarogoulidis, Antonios Sakkas, Stylianos Kakolyris, Haidong Huang |
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| Rok vydání: | 2014 |
| Předmět: |
Pathology
medicine.medical_specialty suicide gene therapy chemistry.chemical_compound Adenosine deaminase melanoma Medicine 5-fluorocytosine Viability assay retinal biology business.industry Melanoma Cytosine deaminase Retinal Suicide gene medicine.disease Adenosine Oncology chemistry Apoptosis Cancer research biology.protein business medicine.drug Research Paper |
| Zdroj: | Journal of Cancer |
| ISSN: | 1837-9664 |
| Popis: | Local treatment as a treatment modality is gaining increased general acceptance over time. Novel drugs and methodologies of local administration are being investigated in an effort to achieve disease local control. Suicide gene therapy is a method that has been investigated as a local treatment with simultaneously distant disease control. In our current experiment we purchased HTB-70 (melanoma cell line, derived from metastatic axillary node) and CRL-2302 (human retinal epithelium) were from ATCC LGC Standards and Ancotil(®), 2.5 g/250 ml (1 g/00ml) (5-Flucytosine) MEDA; Pharmaceuticals Ltd. UK. Adenosine Cytosine Deaminase (Ad.CD) was also used in order to convert the pro-drug 5-Flucytosine to the active 5-Fluoracil. Three different concentrations of 5-Flucytosine (5-FC) were administered (0.2ml, 0.8ml and 1.2ml). At indicated time-points (4h, 8h and 24h) cell viability and apoptosis were measured. Our concept was to investigate whether suicide gene therapy with Ad. CD-5-FC could be used with safety and efficiency as a future local treatment for melanoma located in the eye cavity. Indeed, our results indicated that in every 5-FC administration had mild cytotoxicity for the retinal cells, while increased apoptosis was observed for the melanoma cell line. |
| Databáze: | OpenAIRE |
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